BCHM 307 – Exam 1 | Purdue University 2025/2026 | 80+ Verified Q&A | DNA/RNA Structure, Protein Folding, Buffers, Amino Acids, Thermodynamics, Hydrophobic Effect

This detailed and highly accurate resource contains 80+ exam-style questions with verified answers, specifically designed for students enrolled in BCHM 307 – Biochemistry at Purdue University for the 2025/2026 academic year. Perfectly aligned with foundational biochemistry topics, this guide is essential for preparing for Exam 1, ensuring mastery of biochemical principles and exam success. Key concepts featured include: DNA/RNA composition and replication (e.g., base pairs, hydrogen bonding, 5' and 3' ends, Chargaff's Rule) Protein structure (primary, secondary, tertiary, quaternary, disulfide bonds, helix-breaking residues like Proline) Water chemistry and hydrophobic interactions (hydrophobic effect, amphiphilic molecules, micelles, hydrogen bonding) Thermodynamics and entropy (ΔG, spontaneity, metabolic coupling) pH, pKa, buffers and titration systems (phosphate buffer, bicarbonate-carbonic acid system) Amino acids and peptide bonds (ionizable side chains, thiol groups, phosphorylation, condensation reactions) Cell structures and biomolecular classification (eukaryotes vs prokaryotes, macromolecule polymers, organelles) Myoglobin vs Hemoglobin (oxygen binding curves, allosteric regulation, heme group coordination) Experimental models and genetic understanding (e.g., genomic libraries, nucleotide polymerization, transcription and translation basics) Each question is formatted in a quiz-style layout with the correct answer clearly marked and explained, making it ideal for practice, memorization, and active recall sessions. Recommended for: Undergraduate students in BCHM 307 or equivalent introductory biochemistry courses Pre-med and life science majors at Purdue and similar universities Tutors, TAs, and professors preparing assessments or review content Students preparing for MCAT, GRE Biology, or graduate bioscience coursework This document is a perfect supplement to lectures, textbooks, and lab prep, offering students a reliable way to reinforce biochemical knowledge and perform confidently on assessments. Keywords: BCHM 307 Purdue exam, biochemistry practice questions, protein structure quiz, DNA and RNA bases, amino acid functional groups, peptide bond formation, hemoglobin vs myoglobin, entropy and free energy, pKa and pH buffering, hydrophobic effect biology, Chargaff rule, amphiphilic molecules, allosteric proteins