ASCP MLT Chemistry UPDATED
ACTUAL Exam Questions and CORRECT
Answers
Which element in proteins differentiates substance from carbohydrates and lipids? - CORRECT
ANSWER - nitrogen
What proteins is soluble in water? - CORRECT ANSWER - albumin
How are amino acids joined together to form a chain like structure? - CORRECT
ANSWER - peptide bonding
Proteins may become denatured when subjected to? - CORRECT ANSWER - mechanical
agitation, heat, chemical treatments
What does denaturation of proteins refer to? - CORRECT ANSWER - alteration in tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - CORRECT
ANSWER - cystinuria
How many immunoglobulin classes are currently recognized? - CORRECT ANSWER -
five
which reagent is employed in the serum protein determination? - CORRECT ANSWER -
biuret
What should hemolyzed serum not be used for total protein analysis? - CORRECT
ANSWER - hemoglobin is absorbed at the same wavelength as protein in the reagent
,What is NOT true of the biuret reaction? - CORRECT ANSWER - is relatively free from
interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
CORRECT ANSWER - there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - CORRECT ANSWER -
albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen
in which disease state? - CORRECT ANSWER - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin,
and 45% gamma-globulin, then what condition may exist in the patient? - CORRECT
ANSWER - monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - CORRECT
ANSWER - carbohydrates
Name some glycoproteins? - CORRECT ANSWER - ceruloplasmin, follicle-stimulating
hormone, cryoglobulin
What applies to cryoglobulins? - CORRECT ANSWER - they are temperature-sensitive
proteins
What is true of albumen? - CORRECT ANSWER - it is produced in the liver and it is
water soluble
Decreased alpha-fetoprotein values are associated with what condition? - CORRECT
ANSWER - Down Syndrome
, What protein transports iron? - CORRECT ANSWER - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - CORRECT ANSWER -
blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - CORRECT
ANSWER - ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxime
method? - CORRECT ANSWER - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - CORRECT
ANSWER - elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - CORRECT ANSWER - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and which
reagent? - CORRECT ANSWER - titan yellow
The creatinine clearance test is based on what assumption? - CORRECT ANSWER -
Creatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? -
CORRECT ANSWER - creatinine
Creatinine clearance formula - CORRECT ANSWER - UV/P
or UV/P (1.73/correction factor)
1440 mls/min
ACTUAL Exam Questions and CORRECT
Answers
Which element in proteins differentiates substance from carbohydrates and lipids? - CORRECT
ANSWER - nitrogen
What proteins is soluble in water? - CORRECT ANSWER - albumin
How are amino acids joined together to form a chain like structure? - CORRECT
ANSWER - peptide bonding
Proteins may become denatured when subjected to? - CORRECT ANSWER - mechanical
agitation, heat, chemical treatments
What does denaturation of proteins refer to? - CORRECT ANSWER - alteration in tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - CORRECT
ANSWER - cystinuria
How many immunoglobulin classes are currently recognized? - CORRECT ANSWER -
five
which reagent is employed in the serum protein determination? - CORRECT ANSWER -
biuret
What should hemolyzed serum not be used for total protein analysis? - CORRECT
ANSWER - hemoglobin is absorbed at the same wavelength as protein in the reagent
,What is NOT true of the biuret reaction? - CORRECT ANSWER - is relatively free from
interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
CORRECT ANSWER - there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - CORRECT ANSWER -
albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen
in which disease state? - CORRECT ANSWER - acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin,
and 45% gamma-globulin, then what condition may exist in the patient? - CORRECT
ANSWER - monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - CORRECT
ANSWER - carbohydrates
Name some glycoproteins? - CORRECT ANSWER - ceruloplasmin, follicle-stimulating
hormone, cryoglobulin
What applies to cryoglobulins? - CORRECT ANSWER - they are temperature-sensitive
proteins
What is true of albumen? - CORRECT ANSWER - it is produced in the liver and it is
water soluble
Decreased alpha-fetoprotein values are associated with what condition? - CORRECT
ANSWER - Down Syndrome
, What protein transports iron? - CORRECT ANSWER - transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - CORRECT ANSWER -
blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - CORRECT
ANSWER - ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxime
method? - CORRECT ANSWER - The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - CORRECT
ANSWER - elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - CORRECT ANSWER - Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and which
reagent? - CORRECT ANSWER - titan yellow
The creatinine clearance test is based on what assumption? - CORRECT ANSWER -
Creatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? -
CORRECT ANSWER - creatinine
Creatinine clearance formula - CORRECT ANSWER - UV/P
or UV/P (1.73/correction factor)
1440 mls/min
