CG(ASCP) (Cytogenetics Technologist, American Society for Clinical Pathology)
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
The most important reason why biolog-
ical safety cabinets are used instead of to protect the technologist who is pro-
horizontal laminar flow hoods in the han- cessing the specimens
dling of human tissues is
In which of the following situations would
culture media is turbid in appearance
one suspect bacterial contamination
Which of the following chemicals is used
dimethyl sulfoxide (DMSD)
for cryopreservation?
Which of the following is a buffer used in
HEPES
cultures?
In order for the fragile X to be ex-
pressed in affected individuals, lympho-
folic acid deficient
cytes must be grown in what type of me-
dia?
The following enzymes cab be used to
dissociate Chronic Villus samples (CVS) RNAse
except for
Which of the following is not a necessary
ingredient which is added to modified antibiotics
medium?
All of the following are factors which af-
fect the cryopreservation except
A fast growing culture shows signs of cell
cell confluency: too many or too few cells
death. What is a possible reason for this?
collagenase, pronase, and trypsin are
used to disperse tissue into single-cell proteolytic enzymes
suspension. These substances are:
When harvesting peripheral blood cul-
tures, asceptic technique no longer mat- colcemid
ters following incubation with:
Which of the following culture processes
mitomycin-C stress test of peripheral
would be most useful for identifying pa-
blood
tients w/ fanconi anemia?
, CG(ASCP) (Cytogenetics Technologist, American Society for Clinical Pathology)
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
To avoid potential culture failure, it is
important to monitor the reagents used.
Recording which of the following infor- expiration date
mation will be most important when doc-
umenting culture failure?
The morning after being set-up, a sin-
gle dish of amniotic fluid culture turns
cloudy and appears contaminated. None
harvest the culture as soon as possible
of the other cultures set up that day ap-
pear contaminated. Which of the follow-
ing should be done first?
If an amniotic fluid culture from the same
sample grows well in a different incu-
incubator failure
bator, the probable cause of the culture
failure is
Positive staining w/ Hoechst 33258 re-
veals micronuclei. Which of the following
mycoplasma
agents caused the culture contamina-
tion?
Which of the following statements is true is is useful in removing cells from culture
about trypsin> flasks
Which of the following is the best tis-
sue to use for both a good success
chorionic villi
rate and abnormality detection in spon-
taneous abortion specimen
When a culture becomes contaminated,
the culture medium shifts to an acidic pH.
If the medium contains a pH indicator, yellow
the color of the medium in the flask will
appear
Which of the following reagents is used
in culturing peripheral blood, but not in
phytohemagglutinin (PHA)
culturing bone marrow or amniotic fluid
or chromosome analysis?
BM cycline
, CG(ASCP) (Cytogenetics Technologist, American Society for Clinical Pathology)
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
Which of the following is the best agent
to use for the presence of mycoplasma
growth in a culture?
A bone marrow culture on a patient w/
APL M3 has failed to yield analyzable
metaphases. The culture had a cell den-
sity of 1x10^4 calls/ml. The specimen too much time in transit
had been in transit for 2 days and was
not sent in a transport medium. The most
likely cause of the failure is:
Which of the following microscopes
should be used when cleaning a chori- dissection
onic villus specimen?
Blood specimen for chromosome analy-
sis may be kept in culture up to how 96 hours
many hours after culture initiation?
which of the following cell types is diag-
nostically most problematic if present in maternal decidua
a CVS sample?
Cells from a skin fibroblast culture are
to be frozen for storage in liquid nitro-
gen. The cell concentration is 2X10^7
5
cells/ml. How many 2ml vials can be
frozen if the final cell concentration for
storage is to be 2X10^6?
The time that a flask culture takes to
reach confluency depends on all of the humidity of the culture environment
following except the
Which of the following is true regarding there is less chance of microbial contam-
closed-system cultures? ination
A blood culture for a patient diagnosed
w/ Di-George syndrome fails to grow in that an insufficient number of cells were
culture. The most likely cause of the fail- seeded in the culture
ure is:
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
The most important reason why biolog-
ical safety cabinets are used instead of to protect the technologist who is pro-
horizontal laminar flow hoods in the han- cessing the specimens
dling of human tissues is
In which of the following situations would
culture media is turbid in appearance
one suspect bacterial contamination
Which of the following chemicals is used
dimethyl sulfoxide (DMSD)
for cryopreservation?
Which of the following is a buffer used in
HEPES
cultures?
In order for the fragile X to be ex-
pressed in affected individuals, lympho-
folic acid deficient
cytes must be grown in what type of me-
dia?
The following enzymes cab be used to
dissociate Chronic Villus samples (CVS) RNAse
except for
Which of the following is not a necessary
ingredient which is added to modified antibiotics
medium?
All of the following are factors which af-
fect the cryopreservation except
A fast growing culture shows signs of cell
cell confluency: too many or too few cells
death. What is a possible reason for this?
collagenase, pronase, and trypsin are
used to disperse tissue into single-cell proteolytic enzymes
suspension. These substances are:
When harvesting peripheral blood cul-
tures, asceptic technique no longer mat- colcemid
ters following incubation with:
Which of the following culture processes
mitomycin-C stress test of peripheral
would be most useful for identifying pa-
blood
tients w/ fanconi anemia?
, CG(ASCP) (Cytogenetics Technologist, American Society for Clinical Pathology)
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
To avoid potential culture failure, it is
important to monitor the reagents used.
Recording which of the following infor- expiration date
mation will be most important when doc-
umenting culture failure?
The morning after being set-up, a sin-
gle dish of amniotic fluid culture turns
cloudy and appears contaminated. None
harvest the culture as soon as possible
of the other cultures set up that day ap-
pear contaminated. Which of the follow-
ing should be done first?
If an amniotic fluid culture from the same
sample grows well in a different incu-
incubator failure
bator, the probable cause of the culture
failure is
Positive staining w/ Hoechst 33258 re-
veals micronuclei. Which of the following
mycoplasma
agents caused the culture contamina-
tion?
Which of the following statements is true is is useful in removing cells from culture
about trypsin> flasks
Which of the following is the best tis-
sue to use for both a good success
chorionic villi
rate and abnormality detection in spon-
taneous abortion specimen
When a culture becomes contaminated,
the culture medium shifts to an acidic pH.
If the medium contains a pH indicator, yellow
the color of the medium in the flask will
appear
Which of the following reagents is used
in culturing peripheral blood, but not in
phytohemagglutinin (PHA)
culturing bone marrow or amniotic fluid
or chromosome analysis?
BM cycline
, CG(ASCP) (Cytogenetics Technologist, American Society for Clinical Pathology)
Verified Multiple Choice and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers>Guaranteed Pass!!Current Update
Which of the following is the best agent
to use for the presence of mycoplasma
growth in a culture?
A bone marrow culture on a patient w/
APL M3 has failed to yield analyzable
metaphases. The culture had a cell den-
sity of 1x10^4 calls/ml. The specimen too much time in transit
had been in transit for 2 days and was
not sent in a transport medium. The most
likely cause of the failure is:
Which of the following microscopes
should be used when cleaning a chori- dissection
onic villus specimen?
Blood specimen for chromosome analy-
sis may be kept in culture up to how 96 hours
many hours after culture initiation?
which of the following cell types is diag-
nostically most problematic if present in maternal decidua
a CVS sample?
Cells from a skin fibroblast culture are
to be frozen for storage in liquid nitro-
gen. The cell concentration is 2X10^7
5
cells/ml. How many 2ml vials can be
frozen if the final cell concentration for
storage is to be 2X10^6?
The time that a flask culture takes to
reach confluency depends on all of the humidity of the culture environment
following except the
Which of the following is true regarding there is less chance of microbial contam-
closed-system cultures? ination
A blood culture for a patient diagnosed
w/ Di-George syndrome fails to grow in that an insufficient number of cells were
culture. The most likely cause of the fail- seeded in the culture
ure is:
