his Phlebotomy Certification Exam Study Guide offers215
T
comprehensive questions and direct answers, meticulously
covering essential phlebotomy procedures, safety
protocols, lab tests, and professional standards. Designed
for clarity, it's an invaluable resource for exam success.
omprehensive Study Guide Phlebotomy
C
Certification Exam
1 . What are the key guidelines for performing a capillary puncture?
Perform on the 3rd or 4th finger for adults, heel stick for infants; Lancet cut
across ridges; hold the tube horizontal when filling. Don't squeeze finger too
tightly or milk the finger. Wipe off 1st drop.
. What can cause a vein to collapse during a blood draw?If you pull on the
2
plunger of the syringe too hard or too quickly during a draw or you use too
strong of a vacuum for the vein, this can happen.
. What is an anticoagulant?An agent used to prevent blood clotting and to
3
keep the specimen whole blood.
, . Describe the characteristics and uses of a Red top tube.NO ADDITIVE
4
--- Clot Forms ----Serum used for Chemistries, Electrolytes, Thyroids (TSH,
T4), Hormone, Liver, Kidney, Lipid studies, CMP, BMP.
. Describe the characteristics and uses of a Tiger top (Red/Gray top)
5
tube.Clot activator additive - Clot Forms - SERUM STUDIES.
. Describe the characteristics and uses of a Lavender top tube, including
6
important handling instructions.EDTA additive - No Clot - WHOLE BLOOD
for: Complete blood count (CBC), Blood smear, Blood banking (ABO/Rh), ESR,
HCT, Hmg, Glyco Alc.Must be filled sufficiently and inverted (gentle
rocked) several times.
. Describe the characteristics and uses of a Gold Top tube.Clot activator
7
additive - Clot Forms - SERUM Studies.
. Describe the characteristics and uses of a Grey top tube.Sodium
8
Fluoride/Potassium oxalate - Glucose, Alcohol, FBS.
. Describe the characteristics and uses of a Light blue top tube,
9
including important handling instructions.Sodium citrate additive - No Clot
- Used for coagulation studies (PT, PTT)Tube must be filled to the proper
level and inverted (gentle rocked) several times.
1 0. Why is it important to wipe away the first drop of blood during a
capillary puncture?To remove tissue juices, skin cells, alcohol, contaminates,
avoid activating clotting process, avoid producing incorrect results, avoid
diluting specimen.
1 1. What veins are in the antecubital area?The basilic, cephalic, and the
median.
1 2. What is hemoconcentration, and what can cause it?This is the increase
of the cellular/solid elements in direct ratio to plasma. This can be caused by
"pumping of the fist" and begins after the tourniquet is left on longer than 1
minute.
13. What is Provodine/iodine used for in phlebotomy?Agent used on the
, skin to sterilize the area.
1 4. What is a tourniquet in the context of phlebotomy?Elastic device used
to restrict blood flow to distend veins for phlebotomy.
1 5. When is a Wing infusion set (butterfly) typically used?For small or
fragile veins or difficult veins.
1 6. What does the solid portion of blood contain?Erythrocytes, leukocytes,
and Thrombocytes (Platelets).
1 7. What is plasma?The liquid portion of whole blood; contains all clotting
factors.
1 8. What is serum?The liquid portion of clotted blood; contains NO clotting
factors.
1 9. Where should the tourniquet be placed in regards to the draw site, and
how long should it be left on?3-4 inches above the puncture site. 1 minute.
0. What should the MA do if the patient begins to feel dizzy during the
2
blood draw?Immediately remove the tourniquet and needle and stop the
patient from falling. Lower the patient's head and arms. Put something cold on
the back of the neck.
1. What should the Phlebotomist do after the blood has been
2
successfully drawn and the needle has been properly disposed of?Label
tube, bandage patient, document the draw.
2. WBC, RBC, Hct, Hemoglobin (Hb), RBC indices (MCV, MCH, MCHC),
2
Blood smears, WBC Differential, Platelet count are all considered what
kind of test?Complete Blood Count.
3. Prothrombin time (PT) and Partial thromboplastin time (PTT) are
2
considered what kind of test?Coagulation studies.
4. Cholesterol, Triglycerides, HDL, LDL, are considered what kind of test?
2
Lipid Studies.
25. Sodium, Potassium, Chloride, CO2, Glucose, BUN, Creatinine, Calcium,
T
comprehensive questions and direct answers, meticulously
covering essential phlebotomy procedures, safety
protocols, lab tests, and professional standards. Designed
for clarity, it's an invaluable resource for exam success.
omprehensive Study Guide Phlebotomy
C
Certification Exam
1 . What are the key guidelines for performing a capillary puncture?
Perform on the 3rd or 4th finger for adults, heel stick for infants; Lancet cut
across ridges; hold the tube horizontal when filling. Don't squeeze finger too
tightly or milk the finger. Wipe off 1st drop.
. What can cause a vein to collapse during a blood draw?If you pull on the
2
plunger of the syringe too hard or too quickly during a draw or you use too
strong of a vacuum for the vein, this can happen.
. What is an anticoagulant?An agent used to prevent blood clotting and to
3
keep the specimen whole blood.
, . Describe the characteristics and uses of a Red top tube.NO ADDITIVE
4
--- Clot Forms ----Serum used for Chemistries, Electrolytes, Thyroids (TSH,
T4), Hormone, Liver, Kidney, Lipid studies, CMP, BMP.
. Describe the characteristics and uses of a Tiger top (Red/Gray top)
5
tube.Clot activator additive - Clot Forms - SERUM STUDIES.
. Describe the characteristics and uses of a Lavender top tube, including
6
important handling instructions.EDTA additive - No Clot - WHOLE BLOOD
for: Complete blood count (CBC), Blood smear, Blood banking (ABO/Rh), ESR,
HCT, Hmg, Glyco Alc.Must be filled sufficiently and inverted (gentle
rocked) several times.
. Describe the characteristics and uses of a Gold Top tube.Clot activator
7
additive - Clot Forms - SERUM Studies.
. Describe the characteristics and uses of a Grey top tube.Sodium
8
Fluoride/Potassium oxalate - Glucose, Alcohol, FBS.
. Describe the characteristics and uses of a Light blue top tube,
9
including important handling instructions.Sodium citrate additive - No Clot
- Used for coagulation studies (PT, PTT)Tube must be filled to the proper
level and inverted (gentle rocked) several times.
1 0. Why is it important to wipe away the first drop of blood during a
capillary puncture?To remove tissue juices, skin cells, alcohol, contaminates,
avoid activating clotting process, avoid producing incorrect results, avoid
diluting specimen.
1 1. What veins are in the antecubital area?The basilic, cephalic, and the
median.
1 2. What is hemoconcentration, and what can cause it?This is the increase
of the cellular/solid elements in direct ratio to plasma. This can be caused by
"pumping of the fist" and begins after the tourniquet is left on longer than 1
minute.
13. What is Provodine/iodine used for in phlebotomy?Agent used on the
, skin to sterilize the area.
1 4. What is a tourniquet in the context of phlebotomy?Elastic device used
to restrict blood flow to distend veins for phlebotomy.
1 5. When is a Wing infusion set (butterfly) typically used?For small or
fragile veins or difficult veins.
1 6. What does the solid portion of blood contain?Erythrocytes, leukocytes,
and Thrombocytes (Platelets).
1 7. What is plasma?The liquid portion of whole blood; contains all clotting
factors.
1 8. What is serum?The liquid portion of clotted blood; contains NO clotting
factors.
1 9. Where should the tourniquet be placed in regards to the draw site, and
how long should it be left on?3-4 inches above the puncture site. 1 minute.
0. What should the MA do if the patient begins to feel dizzy during the
2
blood draw?Immediately remove the tourniquet and needle and stop the
patient from falling. Lower the patient's head and arms. Put something cold on
the back of the neck.
1. What should the Phlebotomist do after the blood has been
2
successfully drawn and the needle has been properly disposed of?Label
tube, bandage patient, document the draw.
2. WBC, RBC, Hct, Hemoglobin (Hb), RBC indices (MCV, MCH, MCHC),
2
Blood smears, WBC Differential, Platelet count are all considered what
kind of test?Complete Blood Count.
3. Prothrombin time (PT) and Partial thromboplastin time (PTT) are
2
considered what kind of test?Coagulation studies.
4. Cholesterol, Triglycerides, HDL, LDL, are considered what kind of test?
2
Lipid Studies.
25. Sodium, Potassium, Chloride, CO2, Glucose, BUN, Creatinine, Calcium,
