STRAIGHTERLINE MICROBIOLOGY
LAB BIO250L LABS EXAM 2025
QUESTIONS AND ANSWERS
What is the objective of air exposure? - ANS to demonstrate that microorganism are around
us
define the medium? - ANS liquid or gel designed to expose bacteria
what are the two types if medium and what do they provide? - ANS broth and agar; they
provide variable growth conditions in the form of growth and stability
what three ways do you handle media? - ANS make sure that the top is on the bottom; plate
is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
Copyright ©2025 BRIGHTSTARS ALL RIGHTS RESERVED 1
, define agar - ANS chemical derived from seaweed that solidifies into a jelly-like substance
and becomes a solid
what are two types of agar? - ANS plates and slants
example of agar? - ANS TSA--- trypticase soy agar
what is the air exposure procedure? - ANS open the plate to expose it to the air for several
minutes then seal with parafilm and place in an incubate at 22C for 24 hours then 4C until the
next week
what is the objective of a surface swab? - ANS to demonstrate that their are microorganism
and can only kill some
what is sterilization and some examples? - ANS when you kill all the bacteria;
autoclave and radiation
what is autoclave? - ANS example of sterilization when you use heat and pressure
Copyright ©2025 BRIGHTSTARS ALL RIGHTS RESERVED 2
LAB BIO250L LABS EXAM 2025
QUESTIONS AND ANSWERS
What is the objective of air exposure? - ANS to demonstrate that microorganism are around
us
define the medium? - ANS liquid or gel designed to expose bacteria
what are the two types if medium and what do they provide? - ANS broth and agar; they
provide variable growth conditions in the form of growth and stability
what three ways do you handle media? - ANS make sure that the top is on the bottom; plate
is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
Copyright ©2025 BRIGHTSTARS ALL RIGHTS RESERVED 1
, define agar - ANS chemical derived from seaweed that solidifies into a jelly-like substance
and becomes a solid
what are two types of agar? - ANS plates and slants
example of agar? - ANS TSA--- trypticase soy agar
what is the air exposure procedure? - ANS open the plate to expose it to the air for several
minutes then seal with parafilm and place in an incubate at 22C for 24 hours then 4C until the
next week
what is the objective of a surface swab? - ANS to demonstrate that their are microorganism
and can only kill some
what is sterilization and some examples? - ANS when you kill all the bacteria;
autoclave and radiation
what is autoclave? - ANS example of sterilization when you use heat and pressure
Copyright ©2025 BRIGHTSTARS ALL RIGHTS RESERVED 2
