MCDB 165A Exam Questions and Answers Already Passed Rated A+
Who named cells - Answers Robert Hooke
When did Robert Hooke identify cells? - Answers 1660s
When was the minimal essential medium for cell culture discovered? - Answers 1959
Type of serum commonly used for cell culturing - Answers Fetal bovine serum
What do you use to pick cells up off a plate during passaging and how does it work - Answers trypsin,
protease that digests the proteins helping the cell stick to the plate
What does formalin do? - Answers reacts with side chains on amino acids and puts tissues into stasis,
fixing the tissue
hematoxylin colors ____ ____ - Answers nuclei; blue
eosin colors ____ ____ - Answers cytoplasm; red
dye that fluorescently stains nuclei; interpolating agent, able to slide between bases in DNA - Answers
DAPI
Where does DAPI stain - Answers nucleus
Type of microscopy that uses a pinhole - Answers confocal microscopy
Type of microscopy that uses evanescent wave to view the surface of an object - Answers TIRF
List the components of a membrane phospholipid - Answers two non-polar hydrophobic tails, glycerol,
phosphate, variable group including choline, ethanolamine, serine, or inositol
When did Gunter Blobel develop the signal sequence hypothesis? - Answers 1971
Describe the purpose of Valm et. al - Answers applying systems-level spectral imaging and analysis to
reveal the organelle interactome
How did Valm and colleagues visualize organelles in the cell - Answers fluorescent fusion proteins for
each organelle and a dye for lipid droplets
what two types of microscopy are used in Valm et al - Answers confocal and lattice light sheet
Protein/fluorescent protein combo used in Valm et. al for the lysosome - Answers LAMP1-CFP
protein/fluorescence combo used in Valm et. al for mitochondria - Answers mitochondrial targeting
sequence of cytochrome c oxidase-eGFP
protein/fluorescence combo used in Valm et. al for ER - Answers bovine prolactin fused to YFP and KDEL
, protein/fluorescence combo used in Valm et. al for peroxisome - Answers peroxisome targeting
sequence fused to mOrange2
protein/fluorescence combo used in Valm et. al for Golgi Apparatus - Answers 15 N-terminal amino acids
of sialylytransferase-mApple
type of cells used in Valm et. al - Answers COS-7 African Green Monkey immortalized cell line
List the 5 functions of membranes - Answers 1. Define boundary of cell and serve as permeability layer
2. Organization and localization of function
3. Transport processes
4. Signal detection
5. Cell-to-cell interaction
List the essential components of a plasmid in cloning - Answers Origin of replication, promoter,
selectable parker for antibiotic resistance (against ampicillin), selectable marker to select against cells
that did not take up the plasmid when using eukaryotic organisms (puromycin), multi cloning site,
restriction sites
What media were the cells grown in in Valm et. al - Answers Dulbecco's Modified Eagle's Medium
(DMEM), 10% fetal bovine serum, 5% CO2
What are the 6 steps of an immunofluorescence experiment? - Answers 1. Fixation
2. Permeabilization (poking holes in membrane so antibodies can get through)
3. Blocking (prevent nonspecific binding due to stickiness with serum or dry milk)
4. Primary antibody incubation
5. Secondary antibody incubation
6. Imaging
type of signaling where once cell communicates with another cell at a distance via a blood vessel -
Answers endocrine
____ signaling is usually involved in processes where you want a full body response, such a puberty -
Answers endocrine
type of signaling where one cell communicates to a nearby cell without touching it - Answers paracrine
____ signaling is important for ensuring that cells differentiate properly, such as in the hand to form the
separate fingers - Answers paracrine
Who named cells - Answers Robert Hooke
When did Robert Hooke identify cells? - Answers 1660s
When was the minimal essential medium for cell culture discovered? - Answers 1959
Type of serum commonly used for cell culturing - Answers Fetal bovine serum
What do you use to pick cells up off a plate during passaging and how does it work - Answers trypsin,
protease that digests the proteins helping the cell stick to the plate
What does formalin do? - Answers reacts with side chains on amino acids and puts tissues into stasis,
fixing the tissue
hematoxylin colors ____ ____ - Answers nuclei; blue
eosin colors ____ ____ - Answers cytoplasm; red
dye that fluorescently stains nuclei; interpolating agent, able to slide between bases in DNA - Answers
DAPI
Where does DAPI stain - Answers nucleus
Type of microscopy that uses a pinhole - Answers confocal microscopy
Type of microscopy that uses evanescent wave to view the surface of an object - Answers TIRF
List the components of a membrane phospholipid - Answers two non-polar hydrophobic tails, glycerol,
phosphate, variable group including choline, ethanolamine, serine, or inositol
When did Gunter Blobel develop the signal sequence hypothesis? - Answers 1971
Describe the purpose of Valm et. al - Answers applying systems-level spectral imaging and analysis to
reveal the organelle interactome
How did Valm and colleagues visualize organelles in the cell - Answers fluorescent fusion proteins for
each organelle and a dye for lipid droplets
what two types of microscopy are used in Valm et al - Answers confocal and lattice light sheet
Protein/fluorescent protein combo used in Valm et. al for the lysosome - Answers LAMP1-CFP
protein/fluorescence combo used in Valm et. al for mitochondria - Answers mitochondrial targeting
sequence of cytochrome c oxidase-eGFP
protein/fluorescence combo used in Valm et. al for ER - Answers bovine prolactin fused to YFP and KDEL
, protein/fluorescence combo used in Valm et. al for peroxisome - Answers peroxisome targeting
sequence fused to mOrange2
protein/fluorescence combo used in Valm et. al for Golgi Apparatus - Answers 15 N-terminal amino acids
of sialylytransferase-mApple
type of cells used in Valm et. al - Answers COS-7 African Green Monkey immortalized cell line
List the 5 functions of membranes - Answers 1. Define boundary of cell and serve as permeability layer
2. Organization and localization of function
3. Transport processes
4. Signal detection
5. Cell-to-cell interaction
List the essential components of a plasmid in cloning - Answers Origin of replication, promoter,
selectable parker for antibiotic resistance (against ampicillin), selectable marker to select against cells
that did not take up the plasmid when using eukaryotic organisms (puromycin), multi cloning site,
restriction sites
What media were the cells grown in in Valm et. al - Answers Dulbecco's Modified Eagle's Medium
(DMEM), 10% fetal bovine serum, 5% CO2
What are the 6 steps of an immunofluorescence experiment? - Answers 1. Fixation
2. Permeabilization (poking holes in membrane so antibodies can get through)
3. Blocking (prevent nonspecific binding due to stickiness with serum or dry milk)
4. Primary antibody incubation
5. Secondary antibody incubation
6. Imaging
type of signaling where once cell communicates with another cell at a distance via a blood vessel -
Answers endocrine
____ signaling is usually involved in processes where you want a full body response, such a puberty -
Answers endocrine
type of signaling where one cell communicates to a nearby cell without touching it - Answers paracrine
____ signaling is important for ensuring that cells differentiate properly, such as in the hand to form the
separate fingers - Answers paracrine
