Aseptic Transfer
and Simple Staining of Bacteria
SC165/166 Microbiology lab
, Sterile technique
• “Flame-before-flame-after” sterile technique
• PPE
– Gowns and gloves are required
– All contaminated materials disposed of as Biohazard
• Materials needed for plating/making slides of live
cultures:
– Culture tubes (glass)
– Sterile petri plate or clean glass slide (no coverslip needed)
– Inoculating loop
– General stain
and Simple Staining of Bacteria
SC165/166 Microbiology lab
, Sterile technique
• “Flame-before-flame-after” sterile technique
• PPE
– Gowns and gloves are required
– All contaminated materials disposed of as Biohazard
• Materials needed for plating/making slides of live
cultures:
– Culture tubes (glass)
– Sterile petri plate or clean glass slide (no coverslip needed)
– Inoculating loop
– General stain
