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Instrumental Analysis – Exam 3 Glossary with Verified Answers – Key Terms in Atomic Spectroscopy, Luminescence, and Instrumentation

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This document presents a verified glossary-style compilation of Exam 3 definitions for Instrumental Analysis, emphasizing core vocabulary related to atomic absorption and emission, fluorescence, chemiluminescence, and spectroscopy instrumentation. It includes concise, exam-focused explanations of concepts such as atomization, beam splitters, photodetectors, spectral and chemical interferences, noise types, wavelength selectors, and emission mechanisms. Covering both theoretical and practical aspects, this resource is ideal for reinforcing foundational terminology and preparing for high-level assessments.

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Instrumental Analysis
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Uploaded on
June 6, 2025
Number of pages
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Written in
2024/2025
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Instrumental Analysis Exam 2 (Definitions) questions
with verified answers
Atomization Ans✓✓✓ The process of conversion of a sample into
atoms. Sample is vaporized and mixed with a fuel and then carried into
a flame. This vaporizes the sample and decomposes it into atoms.


Beamsplitter Ans✓✓✓ an optical device which is used to split the
beam of light into 2 or more beams. Can take on many forms such as
cubes, plates, hexagons, pentagons, etc.


Cell Positioning Uncertainty Ans✓✓✓ depends on the cell holder
design. Caused when a cell is not positioned in the same place every
time.


Chemical Interference Ans✓✓✓ alteration of absorption or emission
characteristics of an analyte due to various chemical processes
occurring through atomization


chemiluminescence Ans✓✓✓ process in which a chemical reaction
produces an electronically excited species that emits light as it returns
to its ground state


conventional spectrophotometers Ans✓✓✓ Requires minutes to
record a single spectrum

, Dark Current Ans✓✓✓ small amount of current which flows through a
semiconductor device when it is not operating. Present in all diodes.


Deuterium Lamps (source for UV radiation) Ans✓✓✓ lamp produces
radiation of higher intensity compared to hydrogen lamps. The most
common source in UV.


dissociation Ans✓✓✓ separation of bond due to the absorption of
radiation


Doppler Broadening Ans✓✓✓ the wavelength of radiation emitted or
absorbed by a rapidly moving atom decreases if the motion is toward a
transducer and increases if the motion is away from it.


double beam in space Ans✓✓✓ The beam from the source is split into
two beams by a beam splitter which is a semipermeable mirror


double beam in time Ans✓✓✓ beam from the source is split into two
beams by a chopper and transmitted beam is passed to detector
through a beam splitter which is a semipermeable mirror.


double-beam instruments for absorption Ans✓✓✓ Compares the light
intensity between two light paths, one containing the test sample and
the other is the reference sample. Measurements are easier and more
stable.

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