1|Page
MLT ASCP EXAM NEWEST 2025 ACTUAL EXAM TEST
BANK| COMPLETE 550 REAL EXAM QUESTIONS AND
CORRECT DETAILED ANSWERS (VERIFIED
ANSWERS) ALREADY GRADED A+| ASCP MLT EXAM
PREP 2025|| BRAND NEW!!
What single feature of normal RBCs is most responsible for limiting
their life span?
a. Loss of mitochondria
b. Increased flexibility of the cell membrane
c. Reduction of hemoglobin iron
d. Loss of the nucleus - Correct Answer - d. Loss of the nucleus
Extravascular hemolysis can occur when:
a. RBCs are mechanically ruptured
b. RBCs extravasate for the blood vessels into the tissues
c. Splenic macrophages ingest senescent cells
d. Erythrocytes are trapped in blood clots outside the blood vessels -
Correct Answer - c. Splenic macrophages ingest senescent cells
Which RBC process does not require energy?
a. Oxygen transport
b. Cytoskeletal protein deformability
c. Preventing the peroxidation of proteins and lipids
pg. 1
,2|Page
d. Maintaining cytoplasm cationic electrochemical gradients - Correct
Answer - a. Oxygen transport
What pathyway anaerobically generates energy in the form of ATP?
a. Hexose monophosphate pathway
b. Rapoport-Luebering pathway
c. Embden-Meyerhof pathway
d. 2,3-BPG pathway - Correct Answer - c. Embden-Meyerhof pathway
To survive, the RBC must detoxify peroxides. What hexose-
monophosphate shunt product(s) accomplishes detoxification?
a. ATP
b. 2,3 BPG
c. Pyruvic acid and lactic acid
d. NADPH and reduced glutathione - Correct Answer - d. NADPH and
reduced glutathione
Which of the following helps maintain RBC shape?
a. Membrane phospholipids
b. Cytoskeletal proteins
c. GPI anchor
d. Glycocalyx - Correct Answer - b. Cytoskeletal proteins
A hemoglobin molecule is composed of:
pg. 2
,3|Page
a. One heme molecule and four globin chains
b. Ferrous iron, protoporphyrin IX, and a globin chain
c. Protoporphyrin IX and four globin chains
d. Four heme molecules and four globin chains - Correct Answer - d.
Four heme molecules and four globin chains
Iron is transported in plasma via:
a. Hemosiderin
b. Ferritin
c. Transferrin
d. Hemoglobin - Correct Answer - c. Transferrin
What is the major metabolically available storage form of iron in the
body?
a. Hemosiderin
b. Ferritin
c. Transferrin
d. Hemoglobin - Correct Answer - b. Ferritin
The total iron-binding capacity (TIBC) of the serum is an indirect
measure of which iron-related protein?
a. Hemosiderin
b. Ferritin
c. Transferrin
pg. 3
, 4|Page
d. Hemoglobin - Correct Answer - c. Transferrin
For a patient with classic iron study values that are equivocal for iron
deficiency, which of the following tests would be most helpful in
determining whether iron deficiency is present or not?
a. Zinc protoporphyrin
b. Peripheral blood sideroblast assessment
c. Soluble transferrin receptor
d. Mean cell hemoglobin - Correct Answer - c. Soluble transferrin
receptor
What membrane-associated protein in enterocytes transports iron from
the intestinal lumen into the enterocyte?
a. Transferrin
b. Ferroportin
c. DMT1
d. Ferrochelatase - Correct Answer - c. DMT1
Iron is transported out of macrophages, hepatocytes, and enterocytes by
what membrane protein?
a. Transferrin
b. Ferroportin
c. DMT1
d. Ferrochelatase - Correct Answer - b. Ferroportin
pg. 4
MLT ASCP EXAM NEWEST 2025 ACTUAL EXAM TEST
BANK| COMPLETE 550 REAL EXAM QUESTIONS AND
CORRECT DETAILED ANSWERS (VERIFIED
ANSWERS) ALREADY GRADED A+| ASCP MLT EXAM
PREP 2025|| BRAND NEW!!
What single feature of normal RBCs is most responsible for limiting
their life span?
a. Loss of mitochondria
b. Increased flexibility of the cell membrane
c. Reduction of hemoglobin iron
d. Loss of the nucleus - Correct Answer - d. Loss of the nucleus
Extravascular hemolysis can occur when:
a. RBCs are mechanically ruptured
b. RBCs extravasate for the blood vessels into the tissues
c. Splenic macrophages ingest senescent cells
d. Erythrocytes are trapped in blood clots outside the blood vessels -
Correct Answer - c. Splenic macrophages ingest senescent cells
Which RBC process does not require energy?
a. Oxygen transport
b. Cytoskeletal protein deformability
c. Preventing the peroxidation of proteins and lipids
pg. 1
,2|Page
d. Maintaining cytoplasm cationic electrochemical gradients - Correct
Answer - a. Oxygen transport
What pathyway anaerobically generates energy in the form of ATP?
a. Hexose monophosphate pathway
b. Rapoport-Luebering pathway
c. Embden-Meyerhof pathway
d. 2,3-BPG pathway - Correct Answer - c. Embden-Meyerhof pathway
To survive, the RBC must detoxify peroxides. What hexose-
monophosphate shunt product(s) accomplishes detoxification?
a. ATP
b. 2,3 BPG
c. Pyruvic acid and lactic acid
d. NADPH and reduced glutathione - Correct Answer - d. NADPH and
reduced glutathione
Which of the following helps maintain RBC shape?
a. Membrane phospholipids
b. Cytoskeletal proteins
c. GPI anchor
d. Glycocalyx - Correct Answer - b. Cytoskeletal proteins
A hemoglobin molecule is composed of:
pg. 2
,3|Page
a. One heme molecule and four globin chains
b. Ferrous iron, protoporphyrin IX, and a globin chain
c. Protoporphyrin IX and four globin chains
d. Four heme molecules and four globin chains - Correct Answer - d.
Four heme molecules and four globin chains
Iron is transported in plasma via:
a. Hemosiderin
b. Ferritin
c. Transferrin
d. Hemoglobin - Correct Answer - c. Transferrin
What is the major metabolically available storage form of iron in the
body?
a. Hemosiderin
b. Ferritin
c. Transferrin
d. Hemoglobin - Correct Answer - b. Ferritin
The total iron-binding capacity (TIBC) of the serum is an indirect
measure of which iron-related protein?
a. Hemosiderin
b. Ferritin
c. Transferrin
pg. 3
, 4|Page
d. Hemoglobin - Correct Answer - c. Transferrin
For a patient with classic iron study values that are equivocal for iron
deficiency, which of the following tests would be most helpful in
determining whether iron deficiency is present or not?
a. Zinc protoporphyrin
b. Peripheral blood sideroblast assessment
c. Soluble transferrin receptor
d. Mean cell hemoglobin - Correct Answer - c. Soluble transferrin
receptor
What membrane-associated protein in enterocytes transports iron from
the intestinal lumen into the enterocyte?
a. Transferrin
b. Ferroportin
c. DMT1
d. Ferrochelatase - Correct Answer - c. DMT1
Iron is transported out of macrophages, hepatocytes, and enterocytes by
what membrane protein?
a. Transferrin
b. Ferroportin
c. DMT1
d. Ferrochelatase - Correct Answer - b. Ferroportin
pg. 4
