Cytogenetics ASCP TEST Expected Questions and Answers

Cytogenetics ASCP TEST Expected
Questions and Answers.

Day 1 FISH - ANSWER-1. Denatured in
2xSSC for 2 mins
2. EtOH bath for 2 mins each
(70%,80%,90%)
3. Dry very well

ALL FISH B-CELL - ANSWER--CEP
4,10,17
-t(9;22) BCR/ABL DUAL COLOR/DUAL
FUSION
-t(12;21) ETV6/RUNX1 DUAL COLOR
SINGLE FUSION
-11q23 MLL (rearrangement/deletion
break apart)

ALL FISH T-CELL - ANSWER--t(9;22)
BCR/ABL

, AML FISH - ANSWER--t(8;21)
-INV(16) OR t(16;16)
-11q23 MLL rearrangement/deletion

45, X - ANSWER-Turner syndrome,
webbing of the neck, short stature,
amnorrhea

47, XXY - ANSWER-Klinefelter
syndrome, tall stature, sterility,
gynecomastia

47, XX, +13 - ANSWER-Patau
syndrome, severe mental retardation,
and physical deformities

47, XX,+18 - ANSWER-Edwards
syndrome

del(4)(p16) - ANSWER-Wolf Hirschhorn