* enzymes are effected by environments like weak bonds , plt
-
enzymes have
particular characteristic s ...
catalysts
-
-specificity
-
often require co-factors
allow the formation of a transition State
* VmaxCan't be achieved ,
so there mus be alternative ways to determine what is
happening to an
enzyme/ substrate system
reciprocal - (tm) (2) y m
=
=
Lineweaver-Burk : "Double
slope in t Slope : Im
- X-intercept -I
=
Vmax
km
Vmax
intercept y-intercept 1
6
(42 -y ) m(X2 X)
=
x =
it solving line
-
a :
intercept max ,
f
max
· cheat for solving LWD
-
rocedure for solving Lub
~ make an educated
guess o use
·
Conceptual information to determine
1 solve the line by using minu max IIIII
am
-
.
data points
then use actual equationA
Y2 &X : don't get replaced -only 4,/X,
·
see if those#re plausible
slope is equal
to (distribute m
2 you should
,
get y mx + b equation
=
[Vmax =)
3 X- intercept is when
y 0 (solve for X) look at velocity and it its
platearing
=
.
see
X intercept = -1 and substrate concentration
um
we are
approaching Vmax
4 .
Y intercept is when X =
0 (therefore B) pick a number (easy to divide in 2)
↓
y intercept +
=
Vmax unit/see blu what z chart
poind on
Vimosa
↓
:
correlate km to what substrate Con(
it matches
↓ ↓
a km
how he have Vmax
does it give pick sub
↓
what the Conc use
original equation
you .
data says or
-and
replaces
Close
-
enzymes have
particular characteristic s ...
catalysts
-
-specificity
-
often require co-factors
allow the formation of a transition State
* VmaxCan't be achieved ,
so there mus be alternative ways to determine what is
happening to an
enzyme/ substrate system
reciprocal - (tm) (2) y m
=
=
Lineweaver-Burk : "Double
slope in t Slope : Im
- X-intercept -I
=
Vmax
km
Vmax
intercept y-intercept 1
6
(42 -y ) m(X2 X)
=
x =
it solving line
-
a :
intercept max ,
f
max
· cheat for solving LWD
-
rocedure for solving Lub
~ make an educated
guess o use
·
Conceptual information to determine
1 solve the line by using minu max IIIII
am
-
.
data points
then use actual equationA
Y2 &X : don't get replaced -only 4,/X,
·
see if those#re plausible
slope is equal
to (distribute m
2 you should
,
get y mx + b equation
=
[Vmax =)
3 X- intercept is when
y 0 (solve for X) look at velocity and it its
platearing
=
.
see
X intercept = -1 and substrate concentration
um
we are
approaching Vmax
4 .
Y intercept is when X =
0 (therefore B) pick a number (easy to divide in 2)
↓
y intercept +
=
Vmax unit/see blu what z chart
poind on
Vimosa
↓
:
correlate km to what substrate Con(
it matches
↓ ↓
a km
how he have Vmax
does it give pick sub
↓
what the Conc use
original equation
you .
data says or
-and
replaces
Close
