MODULE 4 EXAM
Actual Questions and Verified Answers
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Portage Learning, Geneva College
Inside you will get:
➢ Module 4 Exam
➢ True & False Questions
➢ Multiple Choice Questions and Answers
➢ Expert-Verified Explanations
,1. A researcher is asked to determine which of two vials contains *E.
coli* and which contains *Salmonella*. Knowing both are Gram-
negative and only one can ferment lactose, which type of media
would be best suited?
Answer choices:
A. Selective media
B. Differential media
C. Enriched media
D. Minimal media
Correct Answer: B. Differential media
Expert Rationale: Differential media such as MacConkey agar incorporate
lactose and a pH indicator, enabling the differentiation of lactose fermenters
(*E. coli*, producing pink colonies) from non-fermenters (*Salmonella*,
producing colorless colonies). This property makes differential media ideal
for distinguishing these two Gram-negative species based on metabolic
activity.
2. True or False: Differential media is best suited for distinguishing
between two similar species of bacteria.
Answer choices:
- True
- False
Correct Answer: True
Expert Rationale: Differential media contain specific indicators (such as
pH indicators or chromogenic substrates) that exploit subtle metabolic
differences between related microbes. Unlike simple growth media,
, differential media enable visualization of phenotypic differences (e.g.,
fermentation of specific sugars), thereby facilitating differentiation of closely
related species.
3. T/F: Growth media is best suited for distinguishing between two
similar species of bacteria.
Answer choices:
- True
- False
Correct Answer: False
Expert Rationale: Growth media are primarily formulated to support the
proliferation of a wide range of microorganisms without exerting selective
pressure. These media contain basic nutrients and do not include
substrates or inhibitors necessary to differentiate between closely related
bacterial species. Therefore, growth media are not appropriate for
distinguishing between similar species because they lack distinguishing
biochemical or physiological indicators.
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4. A researcher needs to detect *Neisseria meningitidis* in a mixed
culture, knowing it is slow growing and other microbes are present.
Which media type would best suit this purpose?
Answer choices:
A. Enriched media
B. Differential media
C. Selective media