Disclaimer: This document was created by department heads with many years of experience teaching the
Pearson Edexcel IGCSE Biology course. We cannot however guarantee that it is an accurate representation
of the Summer 2025 examinations and students should not treat it as such. Please use this as an aid, alongside
other methods of revision.
Pearson Edexcel International GCSE (9-1)
Tuesday 13 May 2025
Afternoon (Time: 2 hours)
Biology
Unit: 4BI1
Paper: 1B
You must have:
Calculator, ruler
Instructions
• Use black ink or a ballpoint pen.
• Answer all questions.
• Answer the questions in the spaces provided.
○ There may be more space than you need.
• Show all the steps in any calculations and state the units.
Information
• The total mark for this paper is 110.
• The marks for each question are shown in brackets.
○ Use this as a guide as to how much time to spend on each question.
Advice
• Read each question carefully before you start to answer it.
• Write your answers neatly and in good English.
• Try to answer every question.
• Check your answers if you have time at the end.
Turn over →
© 2025 Empirical Tuition. All rights reserved.
, Answer all questions.
1. The passage below describes the process of selective breeding.
(a) Complete the passage by writing a suitable word in each blank space. (3)
Selective breeding is a process used to produce organisms with ________________ characteristics. By
mating individuals with these characteristics, their ________________ will inherit them.
For example, wheat has been selectively bred for thousands of years to increase its ________________.
Chickens have been selectively bred over generations to produce larger eggs.
A farmer has recently developed a new breed of chicken called the Golden Layer, which is known for
consistently producing large eggs. All eggs contain protein.
(b) Describe a test that the farmer could use to confirm that his eggs contain protein. Include the
expected result if protein is present. (2)
2
,The farmer wanted to find out more about the nutrients in his new Golden Layer eggs compared to
regular eggs. He sent both types of eggs to a lab for testing. A graph of the results is shown below.
The lab discovered that the Golden Layer eggs contained much higher levels of protein compared to
regular eggs.
(c) Calculate the percentage increase in protein content from the regular eggs to the Golden Layer
eggs. (3)
3
, The farmer made the following statement:
‘Golden Layer eggs are significantly healthier than regular eggs.’
(d) Comment on the farmer’s conclusion. Use the information provided, as well as your own
knowledge. (4)
(Total for Question 1 = 12 marks)
2. Rust fungi are a type of parasitic organism that infect plants.
Rust fungi secrete enzymes that degrade the cell walls found in the leaves of plants. This enables
them to penetrate and feed on plant tissues.
4
Pearson Edexcel IGCSE Biology course. We cannot however guarantee that it is an accurate representation
of the Summer 2025 examinations and students should not treat it as such. Please use this as an aid, alongside
other methods of revision.
Pearson Edexcel International GCSE (9-1)
Tuesday 13 May 2025
Afternoon (Time: 2 hours)
Biology
Unit: 4BI1
Paper: 1B
You must have:
Calculator, ruler
Instructions
• Use black ink or a ballpoint pen.
• Answer all questions.
• Answer the questions in the spaces provided.
○ There may be more space than you need.
• Show all the steps in any calculations and state the units.
Information
• The total mark for this paper is 110.
• The marks for each question are shown in brackets.
○ Use this as a guide as to how much time to spend on each question.
Advice
• Read each question carefully before you start to answer it.
• Write your answers neatly and in good English.
• Try to answer every question.
• Check your answers if you have time at the end.
Turn over →
© 2025 Empirical Tuition. All rights reserved.
, Answer all questions.
1. The passage below describes the process of selective breeding.
(a) Complete the passage by writing a suitable word in each blank space. (3)
Selective breeding is a process used to produce organisms with ________________ characteristics. By
mating individuals with these characteristics, their ________________ will inherit them.
For example, wheat has been selectively bred for thousands of years to increase its ________________.
Chickens have been selectively bred over generations to produce larger eggs.
A farmer has recently developed a new breed of chicken called the Golden Layer, which is known for
consistently producing large eggs. All eggs contain protein.
(b) Describe a test that the farmer could use to confirm that his eggs contain protein. Include the
expected result if protein is present. (2)
2
,The farmer wanted to find out more about the nutrients in his new Golden Layer eggs compared to
regular eggs. He sent both types of eggs to a lab for testing. A graph of the results is shown below.
The lab discovered that the Golden Layer eggs contained much higher levels of protein compared to
regular eggs.
(c) Calculate the percentage increase in protein content from the regular eggs to the Golden Layer
eggs. (3)
3
, The farmer made the following statement:
‘Golden Layer eggs are significantly healthier than regular eggs.’
(d) Comment on the farmer’s conclusion. Use the information provided, as well as your own
knowledge. (4)
(Total for Question 1 = 12 marks)
2. Rust fungi are a type of parasitic organism that infect plants.
Rust fungi secrete enzymes that degrade the cell walls found in the leaves of plants. This enables
them to penetrate and feed on plant tissues.
4
