MT AAB Chemistry questions and answers
with solutions
Which of the four elements in proteins differentiated this class of substances from carbohydrates
and lipids? - ANSWER Nitrogen
Which of the proteins is soluble in water? - ANSWER Albumin
How are amino acids joined together to form a chainlike structure? - ANSWER Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - ANSWER Alteration of tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANSWER Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - ANSWER Maple syrup disease,
phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - ANSWER Five
Which reagent is employed in the serum protein determination? - ANSWER Biuret
Why should hemolyzed serum not be used for total protein analysis? - ANSWER Hemoglobin is
absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - ANSWER It is relatively free from
interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - ANSWER Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages, and is
insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
ANSWER There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWER Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
, Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANSWER 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen
in which disease state? - ANSWER Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? - ANSWER
Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - ANSWER
Carbohydrates
All of the following are glycoproteins: - ANSWER Ceruloplasmin, follicle-stimulating hormone
(FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - ANSWER Cryoglobulin
Which one of the following applies to cryoglobulins? - ANSWER They are temperature-sensitive
proteins
Which of the following statements is true of albumin? - ANSWER It is produced in the liver and
is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - ANSWER Down
syndrome
Which one of the following proteins transports iron? - ANSWER Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - ANSWER Blood urea
nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - ANSWER
Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - ANSWER The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values? -
ANSWER Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - ANSWER Jaffe
with solutions
Which of the four elements in proteins differentiated this class of substances from carbohydrates
and lipids? - ANSWER Nitrogen
Which of the proteins is soluble in water? - ANSWER Albumin
How are amino acids joined together to form a chainlike structure? - ANSWER Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - ANSWER Alteration of tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANSWER Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - ANSWER Maple syrup disease,
phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - ANSWER Five
Which reagent is employed in the serum protein determination? - ANSWER Biuret
Why should hemolyzed serum not be used for total protein analysis? - ANSWER Hemoglobin is
absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - ANSWER It is relatively free from
interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - ANSWER Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages, and is
insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
ANSWER There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWER Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
, Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANSWER 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen
in which disease state? - ANSWER Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? - ANSWER
Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - ANSWER
Carbohydrates
All of the following are glycoproteins: - ANSWER Ceruloplasmin, follicle-stimulating hormone
(FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - ANSWER Cryoglobulin
Which one of the following applies to cryoglobulins? - ANSWER They are temperature-sensitive
proteins
Which of the following statements is true of albumin? - ANSWER It is produced in the liver and
is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - ANSWER Down
syndrome
Which one of the following proteins transports iron? - ANSWER Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - ANSWER Blood urea
nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - ANSWER
Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - ANSWER The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values? -
ANSWER Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - ANSWER Jaffe
