BIO 351 FINAL EXAM QUESTIONS WITH CORRECT ANSWERS| LATEST UPDATE
GUARANTEED SUCCESS
which of the following cues is best suited for promoting juxtacrine signaling? notch or delta
in the signaling diagram on the right, the extracellular ligand binds to a _________. receptor
tyrosine kinase
two mice have all of their spermatogonia killed by treatment with the drug busulfan. Then
healthy spermatogonia are transplanted into the seminiferous tubules of one of the treated
mice. Which of the following will be the most likley outcome of this experiment? the mouse
that had spermatogonia transplanted will restore spermatogenesis, the other will not.
at ovulation, mammalian oocytes are at metaphase II. How is the block released and meiosis
completed? fertilization causes a Ca2+ influx that activates calmodulin which activates
CamKII and calpain. These enzymes then degrade cytostatic factor (CSF)
in the diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
through the ________ ligand on the inducing cell and ___ receptor on the responding cell in
this pathway. delta, notch
which of the following best explains how miR181 decreases the expression of genes?
microRNAS target mRNAS for degradation and block translation
,which of the following properites of ES cells make them useful for making Knock-out
mutations? they are pluripotent, will self-renew, and will integrate vector DNA by
homologous recombination
__ is an important regulator of transcription downstream of Wnt signaling? beta-catenin
mice were made that lacked the gene encoding ZP2. how would you predict that this would
affect fertlization? it would block sperm binding to the oocyte
you want to determine all of the embryonic time points and locations where the Tbx5 gene is
expressed. Which of the following would be the best method to use to do this? Tbx5 in situ
hybridization
if you took skin fibroblasts and increased expression of Oct4, Myc, Klf4, and Sox2, what would
you be converting the fibroblasts into? induced pluripotent stem cells (iPSC)
a transgenic mouse is not a chimera. why? injection of the transgene vector into a fertilized
egg results in all embryonic cells having the transgene
the mouse Pax6 gene with its regulatory regions is shown below. What would be the most likely
result if you generated a targeted mouse mutation (knock in) that destroyed only the sequence
of the transcription factor binding site shown below in bold? transcription of Pax6 in lens,
retina, and neural tube, but not pancreas
,spermatoza must undergo capacitation before they are competent to fertilize oocytes. Which
of the following events are part of capacitation? removal of membrane cholesterol and
activation of adenylate cyclase
differential gene expression is a central theme in development. Which of the following
statements most accurately describes this concept? genomic DNA is the same in all cell
types, but the mRNAs and proteins are different
germline deletion of the Ptpn11 gene leads to embryonic lethality. Which of the following
approaches would be the most useful for deleting this gene specifically in the heart during later
stages? conditional gene knockout
which statement is true? Bindin is necessary for sea urchin sperm to recognize eggs in a
species-specific manner
spermatoza that are harvested for IVF from the epididymis of men with a blockage of their vas
deferens aer incompetent to fertilize eggs. What must first happen for successful fertilization?
capacitation
in the heat map below, each row shows changes in expression of a single mRNA at different
stages of development. What technique was most likely used to generate these data?
transcriptomics
, in your genetics clinic, you overhear the attending physician mention that the delta-like 3
(DLL3) gene was alternately spliced. Which of the following is the most accurate conclusion
based on this statement? there is more than one form of DLL3 mRNA
in the following experiment below you transplant different types of dermal mesenchyme
beneath the epidermal epithelium from the chicken thigh. select the outcomes of each
experiment both feathers and foot scales
which of the following terms describes the process by which the chromosomes of the eff and
those of the sperm combine just before the beginning of the first mitotic division? syngamy
Which of the following adult structures is derived from somites? tendons, vertebrae
Cells that become the _________ undergo a ____________ to __________ transition first. Fill
in the blanks. Sclerotome, epithelial, mesenchymal
The endocardial cushion cells have important roles in heart formation. Defects in the
endocardial cushions have been associated with which of the following? septation defects
Spondylocostal dysostosis and congenital scoliosis are examples of disruption of which of the
following genetic pathways? Notch
GUARANTEED SUCCESS
which of the following cues is best suited for promoting juxtacrine signaling? notch or delta
in the signaling diagram on the right, the extracellular ligand binds to a _________. receptor
tyrosine kinase
two mice have all of their spermatogonia killed by treatment with the drug busulfan. Then
healthy spermatogonia are transplanted into the seminiferous tubules of one of the treated
mice. Which of the following will be the most likley outcome of this experiment? the mouse
that had spermatogonia transplanted will restore spermatogenesis, the other will not.
at ovulation, mammalian oocytes are at metaphase II. How is the block released and meiosis
completed? fertilization causes a Ca2+ influx that activates calmodulin which activates
CamKII and calpain. These enzymes then degrade cytostatic factor (CSF)
in the diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
through the ________ ligand on the inducing cell and ___ receptor on the responding cell in
this pathway. delta, notch
which of the following best explains how miR181 decreases the expression of genes?
microRNAS target mRNAS for degradation and block translation
,which of the following properites of ES cells make them useful for making Knock-out
mutations? they are pluripotent, will self-renew, and will integrate vector DNA by
homologous recombination
__ is an important regulator of transcription downstream of Wnt signaling? beta-catenin
mice were made that lacked the gene encoding ZP2. how would you predict that this would
affect fertlization? it would block sperm binding to the oocyte
you want to determine all of the embryonic time points and locations where the Tbx5 gene is
expressed. Which of the following would be the best method to use to do this? Tbx5 in situ
hybridization
if you took skin fibroblasts and increased expression of Oct4, Myc, Klf4, and Sox2, what would
you be converting the fibroblasts into? induced pluripotent stem cells (iPSC)
a transgenic mouse is not a chimera. why? injection of the transgene vector into a fertilized
egg results in all embryonic cells having the transgene
the mouse Pax6 gene with its regulatory regions is shown below. What would be the most likely
result if you generated a targeted mouse mutation (knock in) that destroyed only the sequence
of the transcription factor binding site shown below in bold? transcription of Pax6 in lens,
retina, and neural tube, but not pancreas
,spermatoza must undergo capacitation before they are competent to fertilize oocytes. Which
of the following events are part of capacitation? removal of membrane cholesterol and
activation of adenylate cyclase
differential gene expression is a central theme in development. Which of the following
statements most accurately describes this concept? genomic DNA is the same in all cell
types, but the mRNAs and proteins are different
germline deletion of the Ptpn11 gene leads to embryonic lethality. Which of the following
approaches would be the most useful for deleting this gene specifically in the heart during later
stages? conditional gene knockout
which statement is true? Bindin is necessary for sea urchin sperm to recognize eggs in a
species-specific manner
spermatoza that are harvested for IVF from the epididymis of men with a blockage of their vas
deferens aer incompetent to fertilize eggs. What must first happen for successful fertilization?
capacitation
in the heat map below, each row shows changes in expression of a single mRNA at different
stages of development. What technique was most likely used to generate these data?
transcriptomics
, in your genetics clinic, you overhear the attending physician mention that the delta-like 3
(DLL3) gene was alternately spliced. Which of the following is the most accurate conclusion
based on this statement? there is more than one form of DLL3 mRNA
in the following experiment below you transplant different types of dermal mesenchyme
beneath the epidermal epithelium from the chicken thigh. select the outcomes of each
experiment both feathers and foot scales
which of the following terms describes the process by which the chromosomes of the eff and
those of the sperm combine just before the beginning of the first mitotic division? syngamy
Which of the following adult structures is derived from somites? tendons, vertebrae
Cells that become the _________ undergo a ____________ to __________ transition first. Fill
in the blanks. Sclerotome, epithelial, mesenchymal
The endocardial cushion cells have important roles in heart formation. Defects in the
endocardial cushions have been associated with which of the following? septation defects
Spondylocostal dysostosis and congenital scoliosis are examples of disruption of which of the
following genetic pathways? Notch
