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Terms in this set (205)
Predominant form of Right-handed B
DNA
DNA and histone Ionic
interaction is due to what
type of bond?
What stains to the minor SYBR Green
groove of DNA duplex?
What is the most Ribosomal RNA (rRNA)
abundant type of RNA?
What does RNA act as in Genome and mRNA
a retrovirus?
What increases half-life 5' Methyl Cap
of mRNA?
What kind of DNA is most Euchromatin
likely to be
transcriptionally active?
What is the most Satellite DNA
common type of
eukaryotic DNA?
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What specimen has the White Blood Cells
most DNA according to
molecular weight?
What amino acids Lysine and Arginine
compose histones?
Amino acid that all Methionine
proteins start with:
What protects the DNA Single-stranded binding protein
strand during
replication?
Leading Strand 3' to 5' template
Lagging Strand 5' to 3' template
What DNA strand is Antisense Strand
being copied in
transcription?
First step after newly Addition of poly(A) tail
formed mRNA is made?
Every time the Telomerase
chromosome is copied it
would become shorter if
not for what enzyme?
What polymerase has Beta/Epsilon
repair activity?
What polymerase acts on Delta
leading strand?
What polymerase acts on Alpha
lagging strand?
Which polymerase is Gamma
used for mitochondrial
replication and repair?
What polymerase binds Topoisomerase
the 5' end to 3' end of
dsDNA?
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Which RNA polymerase RNA Pol II
is mainly for
transcription?
Which polymerase is RNA Pol II
involved with mRNA
synthesis?
What inactivates Diethyl pyrocarbonate (DEPC) or bake glassware
RNAses? for 4 to 6 hours at 400C
What type of restriction Restriction Enzyme Type II
enzyme recognizes
palindromic sites?
What type of restriction Restriction Enzyme Type II
enzyme lacks
methylation activity?
What is necessary to Reverse transcriptase
make cDNA?
Number of SNPs in a 11 billion (10^7)
human genome?
How many genes (base 3 billion base pairs
pairs) are in a human
haploid genome?
The polymerization of Catalyst (APS or TEMED) or Light activation
acrylamide requires:
How does agarose gel Inversely proportional
pore size relate to
concentration?
What is denatured during Hydrogen bonds (inter-strand bonds)
a southern blot?
Melting temperature Tm = (4C x GC Pairs) + (2C x AT pairs)
formula
Which is likely to interfere 5' G
with fluorescent probe?
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