Strasinger AUBF - CSF Ultimate Reviewer
Questions and Answers Verified by Experts
To examine a bloody fluid for the presence of xanthochromia, the fluid should be centrifuged in
a ____ tube Microhematocrit
Xanthochromic supernatant must be examined against a ___ background White
Cell count commonly performed on CSF specimens Leukocyte count
Usually determined only when a traumatic tap has occurred and a correction for leukocytes or
protein is desired RBC count
Can be calculated by performing a total cell count and a WBC count and subtracting the WBC
count from the total count RBC count
Cell count should be performed immediately because WBCs and RBCs begin to lyse within __
hour, 1
,40% of the leukocyte disintegrate after __ hours 2
Normal adult CSF contains ___ WBCs/uL 0 to 5
Normal WBCs in newborn CSF 30 mononuclear cells/uL
Routinely used for performing CSF cell counts Neubauer counting chamber
Not used for performing CSF cell counts, due to high background counts and poor
reproducibility of low counts Electronic cell counters
Increases precision, standardization, and faster turnaroundtime for results Automation
Dilutions for total cell counts are made with _____, mixed by inversion, and loaded into the
hematocytometer Normal saline
Number of cells counted multiplied by the dilution factor equals the ____ Number of cells
per uL
,Used to lyse the RBCs 3% glacial acetic acid
Stains the WBCs providing better differentiation between neutrophils and mononuclear cells
Methylene blue
WBCs are counted in the _____ and the _____ in both sides of the hematocytometer 4
corner square, center square
Used when counting different squares Neubauer formula
All diluents should be checked ____ for contamination by examining them in a counting
chamber under 400x magnification Biweekly
Speed of cytocentrifuge should be checked monthly with a ____ Tachometer
Timing of the cytocentrifuge should be checked with a ____ Stopwatch
, These must be soaked in a bacterial solution for at least 15 minutes, thoroughly rinsed with water
and cleaned with isopropyl alcohol after every use Nondisposable counting chambers
Differential count should be performed on a ____ smear Stained
Sedimentation, filtration, centrifugation and cytocentrifugation Methods for concentration
Cells present in cytometer are forced into a monolayer within a ___ diameter circle on the slide
6mm
Fluid is absorbed by the ____, producing a more concentrated area of the cells Filter paper
blotter
0.1 mL of CSF combined with one drop of ____ produces an adequate cell yield when processed
with the cytocentrifuge 30% albumin
Increases the cell yield and decreases cellular distortion Albumin
Questions and Answers Verified by Experts
To examine a bloody fluid for the presence of xanthochromia, the fluid should be centrifuged in
a ____ tube Microhematocrit
Xanthochromic supernatant must be examined against a ___ background White
Cell count commonly performed on CSF specimens Leukocyte count
Usually determined only when a traumatic tap has occurred and a correction for leukocytes or
protein is desired RBC count
Can be calculated by performing a total cell count and a WBC count and subtracting the WBC
count from the total count RBC count
Cell count should be performed immediately because WBCs and RBCs begin to lyse within __
hour, 1
,40% of the leukocyte disintegrate after __ hours 2
Normal adult CSF contains ___ WBCs/uL 0 to 5
Normal WBCs in newborn CSF 30 mononuclear cells/uL
Routinely used for performing CSF cell counts Neubauer counting chamber
Not used for performing CSF cell counts, due to high background counts and poor
reproducibility of low counts Electronic cell counters
Increases precision, standardization, and faster turnaroundtime for results Automation
Dilutions for total cell counts are made with _____, mixed by inversion, and loaded into the
hematocytometer Normal saline
Number of cells counted multiplied by the dilution factor equals the ____ Number of cells
per uL
,Used to lyse the RBCs 3% glacial acetic acid
Stains the WBCs providing better differentiation between neutrophils and mononuclear cells
Methylene blue
WBCs are counted in the _____ and the _____ in both sides of the hematocytometer 4
corner square, center square
Used when counting different squares Neubauer formula
All diluents should be checked ____ for contamination by examining them in a counting
chamber under 400x magnification Biweekly
Speed of cytocentrifuge should be checked monthly with a ____ Tachometer
Timing of the cytocentrifuge should be checked with a ____ Stopwatch
, These must be soaked in a bacterial solution for at least 15 minutes, thoroughly rinsed with water
and cleaned with isopropyl alcohol after every use Nondisposable counting chambers
Differential count should be performed on a ____ smear Stained
Sedimentation, filtration, centrifugation and cytocentrifugation Methods for concentration
Cells present in cytometer are forced into a monolayer within a ___ diameter circle on the slide
6mm
Fluid is absorbed by the ____, producing a more concentrated area of the cells Filter paper
blotter
0.1 mL of CSF combined with one drop of ____ produces an adequate cell yield when processed
with the cytocentrifuge 30% albumin
Increases the cell yield and decreases cellular distortion Albumin
