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BCH 4024 Exam 4 Questions And Answers
|Latest 2025 | Guaranteed Pass
Humans have "diploid"
genomes with - Answer✔two
complete sets of genes
in every cell.
Prior to division a cell must duplicate its
entire genomic DNA in a process called - Answer✔replication.
Each daughter cell inherits a complete complement of
the genetic information.
A human nucleus:
-- is diameter - Answer✔6 μm
A human nucleus: has - Answer✔6.14 x 109 bp
A human nucleus:at 3.4 Å/bp a human
cell contains - Answer✔~2 m
of DNA!
1|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
DNA polymerase catalyzes the extension of a DNA strand
only in the - Answer✔5'→3' direction
The requirements for DNA
synthesis are: - Answer✔-- a DNA template strand with
a primer (RNA or DNA)
annealed to it. (primer must have a 3'- OH)
-- the reaction substrates are
deoxyribonucleotide
triphosphates.
reaction For DNA synthesis: - Answer✔(dNMP)n + dNTP → (dNMP)n+1 + PPi
DNA polymerase contains - Answer✔Aspartic acid and Mg2+ and ATP to make nucleophilic
attack
The catalytic site for DNA
synthesis is located in the - Answer✔"palm" of the enzyme.
Structure of DNA Polymerase - Answer✔palm
DNA
thumb fingers
Three possible outcomes of DNA synthesis. - Answer✔conservative, semiconservative, non
conservative
Conservative replication
2|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
yields an - Answer✔original intact
DNA molecule and one
entirely newly synthesized
DNA (it doesnt happen this way)
Semiconservative replication
yields - Answer✔two DNA molecules,
each with one parental and one
newly synthesized strand. (the way it works on earth)
Non-conservative replication
yields - Answer✔two newly synthesized
DNA molecules and destruction
of the parental strands. (doesnt work this way)
DNA synthesis
is semiconservative. Product
DNA contains - Answer✔one parental
and one newly synthesized
daughter strand.
"The Meselson and
Stahl Experiment" - Answer✔-E. coli grown in 15N medium
-hybrid DNA 15N-14N with one new stand to original strands (first generation daughter
molecules
-shifted to 14N medium
3|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
-DNA extracted and centrifuges to equilibrium in CsCl density gradient (will float to area of
equal density)
- so 15N the lowest then hybrid then 14N
Leading strand DNA synthesis
occurs - Answer✔continuously 5'→3'.
Lagging strand synthesis
occurs - Answer✔discontinuously
in fragments as a series
of 5'→3' reactions.
-- The products are called
"Okazaki fragments
helicase separates - Answer✔the DNA strands
DNA synthesis begins
at an - Answer✔origin of replication.
Replication forks proceed
in - Answer✔both directions, termed
"bidirectional synthesis
bacteria have what kind of DNA - Answer✔circular
Bidirectional, semiconservative
replication yields . - Answer✔two DNA molecules with
4|Page
BCH 4024 Exam 4 Questions And Answers
|Latest 2025 | Guaranteed Pass
Humans have "diploid"
genomes with - Answer✔two
complete sets of genes
in every cell.
Prior to division a cell must duplicate its
entire genomic DNA in a process called - Answer✔replication.
Each daughter cell inherits a complete complement of
the genetic information.
A human nucleus:
-- is diameter - Answer✔6 μm
A human nucleus: has - Answer✔6.14 x 109 bp
A human nucleus:at 3.4 Å/bp a human
cell contains - Answer✔~2 m
of DNA!
1|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
DNA polymerase catalyzes the extension of a DNA strand
only in the - Answer✔5'→3' direction
The requirements for DNA
synthesis are: - Answer✔-- a DNA template strand with
a primer (RNA or DNA)
annealed to it. (primer must have a 3'- OH)
-- the reaction substrates are
deoxyribonucleotide
triphosphates.
reaction For DNA synthesis: - Answer✔(dNMP)n + dNTP → (dNMP)n+1 + PPi
DNA polymerase contains - Answer✔Aspartic acid and Mg2+ and ATP to make nucleophilic
attack
The catalytic site for DNA
synthesis is located in the - Answer✔"palm" of the enzyme.
Structure of DNA Polymerase - Answer✔palm
DNA
thumb fingers
Three possible outcomes of DNA synthesis. - Answer✔conservative, semiconservative, non
conservative
Conservative replication
2|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
yields an - Answer✔original intact
DNA molecule and one
entirely newly synthesized
DNA (it doesnt happen this way)
Semiconservative replication
yields - Answer✔two DNA molecules,
each with one parental and one
newly synthesized strand. (the way it works on earth)
Non-conservative replication
yields - Answer✔two newly synthesized
DNA molecules and destruction
of the parental strands. (doesnt work this way)
DNA synthesis
is semiconservative. Product
DNA contains - Answer✔one parental
and one newly synthesized
daughter strand.
"The Meselson and
Stahl Experiment" - Answer✔-E. coli grown in 15N medium
-hybrid DNA 15N-14N with one new stand to original strands (first generation daughter
molecules
-shifted to 14N medium
3|Page
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 11:49 AM A+
-DNA extracted and centrifuges to equilibrium in CsCl density gradient (will float to area of
equal density)
- so 15N the lowest then hybrid then 14N
Leading strand DNA synthesis
occurs - Answer✔continuously 5'→3'.
Lagging strand synthesis
occurs - Answer✔discontinuously
in fragments as a series
of 5'→3' reactions.
-- The products are called
"Okazaki fragments
helicase separates - Answer✔the DNA strands
DNA synthesis begins
at an - Answer✔origin of replication.
Replication forks proceed
in - Answer✔both directions, termed
"bidirectional synthesis
bacteria have what kind of DNA - Answer✔circular
Bidirectional, semiconservative
replication yields . - Answer✔two DNA molecules with
4|Page
