AP BIOLOGY LAB 3.1-3.3: ENZYME
STRUCTURE & FUNCTION EXAM
QUESTIONS WITH CORRECT
ANSWERS
For the following questions: Graphs I-IV depict the effect of pH on the activity of four
different hydrolytic enzymes. - ANSWER-II only (it is the graph that increases then
goes "flat")
Gelatin is a protein that is derived from collagen which is found in the bones, skin,
and connective tissue of animals. To investigate the ability of various enzymes to
digest gelatin, a group of students set up an assay involving camera film. Camera
film contains gelatin and appears black when exposed to light but turns clear as the
gelatin gets broken down. The students incubated pieces of exposed camera film in
test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or
amylase) as indicated in Figure 1. The students recorded the time it took for the
enzymes to digest the gelatin in each test tube, turning the film from black to clear.
Which of the following would be the most appropriate control for this experiment? -
ANSWER-A test tube containing a piece of exposed camera film submerged in water
For following group of questions first study the description of the data and then
choose the one best answer to each question following it and fill in the corresponding
oval on the answer sheet.
To study the actions of the enzyme catalase on hydrogen peroxide, students
performed the following experiment. Catalase was extracted from potatoes by
blending raw potatoes in a blender with cold distilled water. The filtrate was stored on
ice. The following hydrogen peroxide solutions were made: 1 percent, 5 percent, 10
percent, and 15 percent. Filter paper disks were soaked in the catalase filtrate and
dropped into beakers containing the various solutions. The activity of the enzyme
was measured by the amount of time it took for the disks to float to the surface of the
solution on the bubbles produced by the reaction. The following data were obtained.
If the potato solution was boiled for 10 minutes and cooled for 10 minu - ANSWER-
more than 30 seconds
During what time interval is the enzyme working at its maximum velocity? -
ANSWER-0-30 seconds
In order to keep the rate constant over the entire time course, which of the following
should be done? - ANSWER-Add more substrate
A student designs an experiment to investigate the effect of environmental pHpH on
amylase function. The design of the experiment is presented in Table 1.
STRUCTURE & FUNCTION EXAM
QUESTIONS WITH CORRECT
ANSWERS
For the following questions: Graphs I-IV depict the effect of pH on the activity of four
different hydrolytic enzymes. - ANSWER-II only (it is the graph that increases then
goes "flat")
Gelatin is a protein that is derived from collagen which is found in the bones, skin,
and connective tissue of animals. To investigate the ability of various enzymes to
digest gelatin, a group of students set up an assay involving camera film. Camera
film contains gelatin and appears black when exposed to light but turns clear as the
gelatin gets broken down. The students incubated pieces of exposed camera film in
test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or
amylase) as indicated in Figure 1. The students recorded the time it took for the
enzymes to digest the gelatin in each test tube, turning the film from black to clear.
Which of the following would be the most appropriate control for this experiment? -
ANSWER-A test tube containing a piece of exposed camera film submerged in water
For following group of questions first study the description of the data and then
choose the one best answer to each question following it and fill in the corresponding
oval on the answer sheet.
To study the actions of the enzyme catalase on hydrogen peroxide, students
performed the following experiment. Catalase was extracted from potatoes by
blending raw potatoes in a blender with cold distilled water. The filtrate was stored on
ice. The following hydrogen peroxide solutions were made: 1 percent, 5 percent, 10
percent, and 15 percent. Filter paper disks were soaked in the catalase filtrate and
dropped into beakers containing the various solutions. The activity of the enzyme
was measured by the amount of time it took for the disks to float to the surface of the
solution on the bubbles produced by the reaction. The following data were obtained.
If the potato solution was boiled for 10 minutes and cooled for 10 minu - ANSWER-
more than 30 seconds
During what time interval is the enzyme working at its maximum velocity? -
ANSWER-0-30 seconds
In order to keep the rate constant over the entire time course, which of the following
should be done? - ANSWER-Add more substrate
A student designs an experiment to investigate the effect of environmental pHpH on
amylase function. The design of the experiment is presented in Table 1.
