Microbiology Lab Practical 1 Study
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Illuminator - ✔✔Filter for shorter λ
Condenser microscope - ✔✔Focuses light into specimen
Diaphragm (iris) - ✔✔Controls amount of light to specimen
Objective lenses at nosepiece - ✔✔Magnifies image 10, 40, or 100 times
Ocular lens - ✔✔magnifies image 10X
Course adjustment - ✔✔focuses specimen in a broad way
Fine adjustment - ✔✔fine focuses specimen
Stage - ✔✔a platform used to hold the slide containing the specimen
Arm - ✔✔serves as a handle for carrying microscope
1
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,Mechanical stage adjuster - ✔✔moves stage forward and back
Stage clip - ✔✔Holds microscope slide in position
Magnification - ✔✔increase in apparent size
Resolution - ✔✔clarity
Oil has the __________ refractive index as glass - ✔✔same
This is the part of the microscope that should not be
touched under high power. - ✔✔the course adjustment knob
How does the light intensity change when a student goes from a low
power objective to a high power objective? - ✔✔The field of vision
narrowed
Which lens do you use oil with? - ✔✔100X lens
How does immersion oil work to enhance resolution? - ✔✔Same refractive
index as glass; prevents loss of light to air
2
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, What is the maximum resolution of your microscope (in micrometers) or
how far apart do two organisms have to be from one another in order to
see them as 2 different organisms under the scope? - ✔✔.2 um with 100X
What is the total magnification of your scope when you use each lens? -
✔✔ocular lens magnification times the magnification of the objective lens
10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? -
✔✔lower the stage
If your cells look washed out under the scope what can you do to fix the
lighting - ✔✔Adjust the lighting by using diaphragm or condenser
Binocular versus monocular - ✔✔Binocular has two eyepieces. Monocular
has one eyepiece.
Inorder to increase the resolution under the 100X oil objective, you must
open this part of the microscope. - ✔✔diaphragm
path of light through a microscope and tell what happens after the light
passes through each part. - ✔✔
3
100% Pass Guarantee Katelyn Whitman, All Rights
guide Questions and Answers 100%
Pass
Illuminator - ✔✔Filter for shorter λ
Condenser microscope - ✔✔Focuses light into specimen
Diaphragm (iris) - ✔✔Controls amount of light to specimen
Objective lenses at nosepiece - ✔✔Magnifies image 10, 40, or 100 times
Ocular lens - ✔✔magnifies image 10X
Course adjustment - ✔✔focuses specimen in a broad way
Fine adjustment - ✔✔fine focuses specimen
Stage - ✔✔a platform used to hold the slide containing the specimen
Arm - ✔✔serves as a handle for carrying microscope
1
100% Pass Guarantee Katelyn Whitman, All Rights
,Mechanical stage adjuster - ✔✔moves stage forward and back
Stage clip - ✔✔Holds microscope slide in position
Magnification - ✔✔increase in apparent size
Resolution - ✔✔clarity
Oil has the __________ refractive index as glass - ✔✔same
This is the part of the microscope that should not be
touched under high power. - ✔✔the course adjustment knob
How does the light intensity change when a student goes from a low
power objective to a high power objective? - ✔✔The field of vision
narrowed
Which lens do you use oil with? - ✔✔100X lens
How does immersion oil work to enhance resolution? - ✔✔Same refractive
index as glass; prevents loss of light to air
2
100% Pass Guarantee Katelyn Whitman, All Rights
, What is the maximum resolution of your microscope (in micrometers) or
how far apart do two organisms have to be from one another in order to
see them as 2 different organisms under the scope? - ✔✔.2 um with 100X
What is the total magnification of your scope when you use each lens? -
✔✔ocular lens magnification times the magnification of the objective lens
10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? -
✔✔lower the stage
If your cells look washed out under the scope what can you do to fix the
lighting - ✔✔Adjust the lighting by using diaphragm or condenser
Binocular versus monocular - ✔✔Binocular has two eyepieces. Monocular
has one eyepiece.
Inorder to increase the resolution under the 100X oil objective, you must
open this part of the microscope. - ✔✔diaphragm
path of light through a microscope and tell what happens after the light
passes through each part. - ✔✔
3
100% Pass Guarantee Katelyn Whitman, All Rights
