MOLECULAR DIAGNOSTICS TEST
QUESTIONS AND ANSWERS
What does DNA Gyrase do in DNA replication? - ANSWER-1) Unwind the helix
2) add protein stabilize
What does RNA Polymerase do in DNA replication? - ANSWER-They make short
RNA primers
What does DNA Polymerase III do in DNA replication? - ANSWER-This add bases
What does DNA Polymerase I do in DNA replication? - ANSWER-1) It digest the
RNA primer
2) Then it add the base
What does the DNA ligase do in DNA replication? - ANSWER-It add the DNA
phosphodiester bonds
What is the result of PCR (polymerase chain reaction)? - ANSWER-1) Technique
that result in Nucleic Acid Amplifiaction of a DNA region.
What is the product of PCR (polymerase chain reaction) and why is called that? -
ANSWER-1) Amplicons
2) During amplification where you take one DNA segment and make a millions
copies.
What is need for amplifications during a PCR in-vitro? - ANSWER-1) Enzymes like
polymerase
2) Primers
3) Master Mix
4) Need to do a series of heating and a series of cooling.
In PCR, what reagents does the master mix contain and why? - ANSWER-1) Tag
(thermus aquatice) which act like DNA polymerase.
2) Deoxynucleoside triphosphate (dNTP) free nucelociteds
3) Primers which give a starting point
4) Buffer and MgCl2 which is use to maintain the pH and Mg 2+ is Cofactor
5) Mineral oil or PCR Gems which subsited by lipid heat. These help prevent
contamination and evapulation.
What are the Requirements to perform a PCR? - ANSWER-1) Specimen form either
a Primary specimen or sub culture
2) Special Area so you can limit some of the contamination because if you get a few
bacterial they will turn into millions.
3) Thermocycle
QUESTIONS AND ANSWERS
What does DNA Gyrase do in DNA replication? - ANSWER-1) Unwind the helix
2) add protein stabilize
What does RNA Polymerase do in DNA replication? - ANSWER-They make short
RNA primers
What does DNA Polymerase III do in DNA replication? - ANSWER-This add bases
What does DNA Polymerase I do in DNA replication? - ANSWER-1) It digest the
RNA primer
2) Then it add the base
What does the DNA ligase do in DNA replication? - ANSWER-It add the DNA
phosphodiester bonds
What is the result of PCR (polymerase chain reaction)? - ANSWER-1) Technique
that result in Nucleic Acid Amplifiaction of a DNA region.
What is the product of PCR (polymerase chain reaction) and why is called that? -
ANSWER-1) Amplicons
2) During amplification where you take one DNA segment and make a millions
copies.
What is need for amplifications during a PCR in-vitro? - ANSWER-1) Enzymes like
polymerase
2) Primers
3) Master Mix
4) Need to do a series of heating and a series of cooling.
In PCR, what reagents does the master mix contain and why? - ANSWER-1) Tag
(thermus aquatice) which act like DNA polymerase.
2) Deoxynucleoside triphosphate (dNTP) free nucelociteds
3) Primers which give a starting point
4) Buffer and MgCl2 which is use to maintain the pH and Mg 2+ is Cofactor
5) Mineral oil or PCR Gems which subsited by lipid heat. These help prevent
contamination and evapulation.
What are the Requirements to perform a PCR? - ANSWER-1) Specimen form either
a Primary specimen or sub culture
2) Special Area so you can limit some of the contamination because if you get a few
bacterial they will turn into millions.
3) Thermocycle
