MCB 150 Final Exam Review Questions
And Answers Updated To Pass.
Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune
system. Some vaccinations are comprised of a weakened live virus and are not recommended for those
who are immunocompromised. What strategies can help protect the immunocompromised individuals in
a community? Choose all that apply.
(A) Achieve high vaccination rates to provide herd immunity.
(B) Vaccinate them even if they are immunocompromised.
(C) Create an alternative non-live vaccine.
(C) Vaccinate their family and close contacts. - Answer A, C, D
What experimental question(s) can be addressed by a microarray analysis? Choose all that apply.
(A) The sequence of a certain gene in an organism
(B) If a certain gene is being expressed in a specific tissue
(C) If a certain gene is being expressed in an organism
(D) If a certain gene is expressed at different levels in different tissues
(E) If a certain gene is expressed in some developmental stages but not others - Answer B, C, D, E
Consider the following statements about genomes:
(1) There is a linear relationship between the size of a eukaryote's genome and the number of protein-
coding genes.
(2) Although prokaryotic genomes are typically smaller than eukaryotic genomes, a higher percentage of
a prokaryote's genome consists of repetitive sequences.
Determine which option below is correct.
(A) Statements 1 and 2 are both true.
(B) Statements 1 and 2 are both false.
,(C) Statement 1 is true, Statement 2 is false.
(D) Statement 1 is false, Statement 2 is true. - Answer B
Which of the following sequences are most likely to be recognized by a Type II restriction enzyme (the
type described in your text)? Only one strand of a double stranded DNA sequence is shown, and all
sequences are given 5'-3'. Choose all that apply.
(A) TTTTTT
(B) AGAGAG
(C) GCAACG
(D) CCCGGG
(E) GATTACA - Answer D
Which of the following are typically used to construct a cDNA library? The list does not necessarily
contain everything needed. Choose all that apply.
(A) DNA ligase
(B) Reverse transcriptase
(C) Whole cell DNA
(D) Primary mRNA transcripts
(E) DNA polymerase - Answer A, B, D, E
You are preparing to use a reverse transcriptase to create cDNA from a sample containing purified
mature eukaryotic mRNA. If you are preparing a primer using only one type of nucleotide, the primer
most likely to be successful would be __________.
(A) poly-A
(B) poly-C
(C) poly-G
(D) poly-T
,(E) No primer composed of a single type of nucleotide can be used to prepare cDNA from these mRNAs. -
Answer D
Identify any mutation that matches all of the following criteria:
Classified as a point mutation
Results in a change in the primary sequence of the protein
Choose all that apply.
(A) Insertions
(B) Deletions
(C) Nonsense
(D) Missense
(E) Same sense
(F) Frameshift - Answer C, D, F
Using information on this website:
https://www.thegenehome.com/how-does-gene-therapy-work/techniques
which type of gene therapy do you feel would be more beneficial for Lee F.?
(A) in vivo
(B) ex vivo - Answer A
HIV enters a cell by _______________ and leaves the cell by _______________.
(A) endocytosis; lysis
(B) nucleic acid injection; lysis
(C) membrane fusion; budding
(D) membrane fusion; lysis
(E) nucleic acid injection; budding - Answer C
, It is necessary for what signal to be present on a molecule of HIV integrase?
(A) ER signal sequence
(B) Nuclear localization signal
(C) Mitochondrial targeting signal
(D) Extracellular matrix binding domain
(E) HIV integrase does not require any of these signals. - Answer B
What is meant by a virus having a segmented genome?
(A) The virus exhibits both a lytic and a lysogenic cycle.
(B) More than one copy of the viral genome is packaged into a capsid.
(C) The entire viral genome is not found on a single nucleic acid molecule.
(D) The genetic material is a combination of RNA and DNA. - Answer C
Scientists have summarized a typical viral replicative cycle into six steps, although not all viruses proceed
through each step. Which step(s) are not found in the replicative cycle of every virus? Choose all that
apply.
(A) Integration
(B) Synthesis of viral components
(C) Release
(D) Entry
(E) Assembly of viral particles
(F) Attachment - Answer A
Mad cow disease is caused by ____________.
And Answers Updated To Pass.
Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune
system. Some vaccinations are comprised of a weakened live virus and are not recommended for those
who are immunocompromised. What strategies can help protect the immunocompromised individuals in
a community? Choose all that apply.
(A) Achieve high vaccination rates to provide herd immunity.
(B) Vaccinate them even if they are immunocompromised.
(C) Create an alternative non-live vaccine.
(C) Vaccinate their family and close contacts. - Answer A, C, D
What experimental question(s) can be addressed by a microarray analysis? Choose all that apply.
(A) The sequence of a certain gene in an organism
(B) If a certain gene is being expressed in a specific tissue
(C) If a certain gene is being expressed in an organism
(D) If a certain gene is expressed at different levels in different tissues
(E) If a certain gene is expressed in some developmental stages but not others - Answer B, C, D, E
Consider the following statements about genomes:
(1) There is a linear relationship between the size of a eukaryote's genome and the number of protein-
coding genes.
(2) Although prokaryotic genomes are typically smaller than eukaryotic genomes, a higher percentage of
a prokaryote's genome consists of repetitive sequences.
Determine which option below is correct.
(A) Statements 1 and 2 are both true.
(B) Statements 1 and 2 are both false.
,(C) Statement 1 is true, Statement 2 is false.
(D) Statement 1 is false, Statement 2 is true. - Answer B
Which of the following sequences are most likely to be recognized by a Type II restriction enzyme (the
type described in your text)? Only one strand of a double stranded DNA sequence is shown, and all
sequences are given 5'-3'. Choose all that apply.
(A) TTTTTT
(B) AGAGAG
(C) GCAACG
(D) CCCGGG
(E) GATTACA - Answer D
Which of the following are typically used to construct a cDNA library? The list does not necessarily
contain everything needed. Choose all that apply.
(A) DNA ligase
(B) Reverse transcriptase
(C) Whole cell DNA
(D) Primary mRNA transcripts
(E) DNA polymerase - Answer A, B, D, E
You are preparing to use a reverse transcriptase to create cDNA from a sample containing purified
mature eukaryotic mRNA. If you are preparing a primer using only one type of nucleotide, the primer
most likely to be successful would be __________.
(A) poly-A
(B) poly-C
(C) poly-G
(D) poly-T
,(E) No primer composed of a single type of nucleotide can be used to prepare cDNA from these mRNAs. -
Answer D
Identify any mutation that matches all of the following criteria:
Classified as a point mutation
Results in a change in the primary sequence of the protein
Choose all that apply.
(A) Insertions
(B) Deletions
(C) Nonsense
(D) Missense
(E) Same sense
(F) Frameshift - Answer C, D, F
Using information on this website:
https://www.thegenehome.com/how-does-gene-therapy-work/techniques
which type of gene therapy do you feel would be more beneficial for Lee F.?
(A) in vivo
(B) ex vivo - Answer A
HIV enters a cell by _______________ and leaves the cell by _______________.
(A) endocytosis; lysis
(B) nucleic acid injection; lysis
(C) membrane fusion; budding
(D) membrane fusion; lysis
(E) nucleic acid injection; budding - Answer C
, It is necessary for what signal to be present on a molecule of HIV integrase?
(A) ER signal sequence
(B) Nuclear localization signal
(C) Mitochondrial targeting signal
(D) Extracellular matrix binding domain
(E) HIV integrase does not require any of these signals. - Answer B
What is meant by a virus having a segmented genome?
(A) The virus exhibits both a lytic and a lysogenic cycle.
(B) More than one copy of the viral genome is packaged into a capsid.
(C) The entire viral genome is not found on a single nucleic acid molecule.
(D) The genetic material is a combination of RNA and DNA. - Answer C
Scientists have summarized a typical viral replicative cycle into six steps, although not all viruses proceed
through each step. Which step(s) are not found in the replicative cycle of every virus? Choose all that
apply.
(A) Integration
(B) Synthesis of viral components
(C) Release
(D) Entry
(E) Assembly of viral particles
(F) Attachment - Answer A
Mad cow disease is caused by ____________.
