pGLO transformation lab Test Questions
With Complete Solutions.
the ability of the cells to live and reproduce in the presence of an antibiotic such as ampicillin - Answer
the cells cannot survive, so they'll die
describe how you could use two LB/agar plates, some e. coli, and some ampicillin to determine how e.
coli cells are affected by ampicillin - Answer - you have to put equal amounts of e. coli on the two
LB/agar plates
- one will contain agar and the other will contain ampicillin
- if there are fewer cells left than there originally were, then you can tell it's e. coli because e. coli is
affected by the ampicillin and that's what kills the cells
what would you expect your experimental results to indicate about the effect of ampicillin on the e. coli
cells? - Answer since ampicillin is an antibiotic, it will kill the bacteria. there would be fewer e. coli cells
when the experiment is done and those that remain are ampicillin resistant
why do we not add plasmid DNA to the -pGLO tube? - Answer there are control plates that will be used
to compare against the +pGLO plates and let us see whether or not any transformations occurred
on which of the plates would you expect to find bacteria most like the original non-transformed e. coli
colonies you initially observed? - Answer -pGLO because it doesn't contain any antibiotics (ampicillin or
arabinose) and therefore, normal bacterial growth will take place
if there are any genetically transformed bacterial cells, which plates would they most likely be located
on? - Answer +pGLO, ampicillin, ara c, and +pGLO amp because it will take up all of the pGLO plasmid,
which expresses the ampicillin resistant gene and makes the GFG gene fluoresce green
which plates should be compared to determine if any genetic transformation has occurred? - Answer
pGLO amp and +pGLO amp because the pGLO acts as the control and shouldn't express the amp
resistant gene
With Complete Solutions.
the ability of the cells to live and reproduce in the presence of an antibiotic such as ampicillin - Answer
the cells cannot survive, so they'll die
describe how you could use two LB/agar plates, some e. coli, and some ampicillin to determine how e.
coli cells are affected by ampicillin - Answer - you have to put equal amounts of e. coli on the two
LB/agar plates
- one will contain agar and the other will contain ampicillin
- if there are fewer cells left than there originally were, then you can tell it's e. coli because e. coli is
affected by the ampicillin and that's what kills the cells
what would you expect your experimental results to indicate about the effect of ampicillin on the e. coli
cells? - Answer since ampicillin is an antibiotic, it will kill the bacteria. there would be fewer e. coli cells
when the experiment is done and those that remain are ampicillin resistant
why do we not add plasmid DNA to the -pGLO tube? - Answer there are control plates that will be used
to compare against the +pGLO plates and let us see whether or not any transformations occurred
on which of the plates would you expect to find bacteria most like the original non-transformed e. coli
colonies you initially observed? - Answer -pGLO because it doesn't contain any antibiotics (ampicillin or
arabinose) and therefore, normal bacterial growth will take place
if there are any genetically transformed bacterial cells, which plates would they most likely be located
on? - Answer +pGLO, ampicillin, ara c, and +pGLO amp because it will take up all of the pGLO plasmid,
which expresses the ampicillin resistant gene and makes the GFG gene fluoresce green
which plates should be compared to determine if any genetic transformation has occurred? - Answer
pGLO amp and +pGLO amp because the pGLO acts as the control and shouldn't express the amp
resistant gene
