BCH3025 Module 3 UF Lehninger Biochemistry 7e Chapter
5 Questions With Complete Solutions
[L] at which half of the available ligand binding sites are
occupied Correct Answers Y = 0.5
corresponds to 1/Ka or Kd
2,3-bisphosphoglycerate (BPG) Correct Answers -released by
RBCs as they break down glucose for energy
-Binds to hemoglobin in the cavity between beta-subunits in the
T state
-increases release of oxygen
-concentration increases in people suffering from hypoxia
A bands Correct Answers dark bands composed of thick
myosin filaments overlapping thin actin filaments
A power stroke involves: Correct Answers -the myosin head
forming a crossbridge with actin and sliding of the thin filament
over the thick filament
-Pi release triggers a "power stroke," a conformational change in
the myosin head that moves actin and myosin filaments relative
to one another.
about what percent of the amino acid residues in the myoglobin
protein can be found in the alpha-helices? Correct Answers
about 78%
actin Correct Answers A globular protein that links into chains,
two of which twist helically about each other, forming
microfilaments in muscle and other contractile elements in cells.
,adult hemoglobin Correct Answers 2 alpha (141 residues each)
and 2 beta chains (146 residues each)
structures are very similar to myoglobin even though fewer than
half of the amino acid residues are identical
helix naming similar to myoglobin except alpha subunit does not
have a short D helix
heme-binding pocket mostly made of E and F helices
all enzymes Correct Answers catalyze reactions
allosteric protein Correct Answers A protein (generally with
multiple subunits) with multiple ligand-binding sites, such that
ligand binding at one site affects ligand binding at another.
an individual amino acid residue is designated either by Correct
Answers -its position in the amino acid sequence (His93)
-its location in the sequence of a particular alpha-helical
segment (His F8)
antibodies Correct Answers Immunoglobulins (Ig) produced by
the immune system in response to bacteria, viruses, or other
antigenic substances.
-make up 20% of bood protein
-generally binds to only a part (epitope) of a large antigen
-The chains are linked by disulfide bonds
antigen Correct Answers a toxin or other foreign substance that
induces an immune response in the body, especially the
production of antibodies.
ex. virus, bacterial cell wall, individual protein, etc
, Antigenic determinant (epitope) Correct Answers specific
region on the antigen that the antibody reacts with
association constant (Ka) Correct Answers equilibrium
constant providing a measure of the affinity of the ligand L for
the protein
higher Ka = higher affinity of the ligand for the protein
Ka = ka/kd = [PL]/[P]x[L]
-can also be determined from a plot of Y v. [L] (free ligand
concentration)
association rate constant Correct Answers ka
units of M-1 s-1
second-order reaction
P + L -> PL
forward reaction forming the PL complex
B lymphocytes (B cells) Correct Answers Lymphocyte that
matures in the bone marrow and produce and secrete antibodies
(immunoglobulins)
binding site Correct Answers the location on a receptor protein
to which a ligand binds
a given protein may have separate binding sites for several
different ligands
binding site and ligand are complementary in.. Correct Answers
size
shape
charge
5 Questions With Complete Solutions
[L] at which half of the available ligand binding sites are
occupied Correct Answers Y = 0.5
corresponds to 1/Ka or Kd
2,3-bisphosphoglycerate (BPG) Correct Answers -released by
RBCs as they break down glucose for energy
-Binds to hemoglobin in the cavity between beta-subunits in the
T state
-increases release of oxygen
-concentration increases in people suffering from hypoxia
A bands Correct Answers dark bands composed of thick
myosin filaments overlapping thin actin filaments
A power stroke involves: Correct Answers -the myosin head
forming a crossbridge with actin and sliding of the thin filament
over the thick filament
-Pi release triggers a "power stroke," a conformational change in
the myosin head that moves actin and myosin filaments relative
to one another.
about what percent of the amino acid residues in the myoglobin
protein can be found in the alpha-helices? Correct Answers
about 78%
actin Correct Answers A globular protein that links into chains,
two of which twist helically about each other, forming
microfilaments in muscle and other contractile elements in cells.
,adult hemoglobin Correct Answers 2 alpha (141 residues each)
and 2 beta chains (146 residues each)
structures are very similar to myoglobin even though fewer than
half of the amino acid residues are identical
helix naming similar to myoglobin except alpha subunit does not
have a short D helix
heme-binding pocket mostly made of E and F helices
all enzymes Correct Answers catalyze reactions
allosteric protein Correct Answers A protein (generally with
multiple subunits) with multiple ligand-binding sites, such that
ligand binding at one site affects ligand binding at another.
an individual amino acid residue is designated either by Correct
Answers -its position in the amino acid sequence (His93)
-its location in the sequence of a particular alpha-helical
segment (His F8)
antibodies Correct Answers Immunoglobulins (Ig) produced by
the immune system in response to bacteria, viruses, or other
antigenic substances.
-make up 20% of bood protein
-generally binds to only a part (epitope) of a large antigen
-The chains are linked by disulfide bonds
antigen Correct Answers a toxin or other foreign substance that
induces an immune response in the body, especially the
production of antibodies.
ex. virus, bacterial cell wall, individual protein, etc
, Antigenic determinant (epitope) Correct Answers specific
region on the antigen that the antibody reacts with
association constant (Ka) Correct Answers equilibrium
constant providing a measure of the affinity of the ligand L for
the protein
higher Ka = higher affinity of the ligand for the protein
Ka = ka/kd = [PL]/[P]x[L]
-can also be determined from a plot of Y v. [L] (free ligand
concentration)
association rate constant Correct Answers ka
units of M-1 s-1
second-order reaction
P + L -> PL
forward reaction forming the PL complex
B lymphocytes (B cells) Correct Answers Lymphocyte that
matures in the bone marrow and produce and secrete antibodies
(immunoglobulins)
binding site Correct Answers the location on a receptor protein
to which a ligand binds
a given protein may have separate binding sites for several
different ligands
binding site and ligand are complementary in.. Correct Answers
size
shape
charge
