Micro, Chpt 3 Key Terms Questions With
Complete Solutions
Culture Correct Ans-A population of microbes on a prepared culture medium conductive to
their growth
Culture media Correct Ans-A prepared substance which contains nutrients to grow microbes.
Types of culture media include broths, gelatins, and agar and may be placed in Petri dishes or
test tubes.
differential media Correct Ans-Types of media used to distinguish particular colonies of
bacteria from other colonies that are growing on the same Petri dish.
enriched media Correct Ans-Either a solid or broth mixture that contains nutrients which
promote the growth of fastidious microbes.
Enzyme Correct Ans-A complex protein with the ability to induce a chemical change with out
being changed itself. When secreted by a bacterium, it invaded the hosts cells and tissues to
produce a reaction including breakdown of substances into simple compounds or causing disease
enzyme-linked immunosorbent assay (ELISA) test Correct Ans-A laboratory test using
antisera to identify a particular bacteria, antibodies, or hormones
, fastidius bacteria Correct Ans-Bacteria which have complex and specific nutritional
requirements for growth and survival
flagella staining Correct Ans-Staining procedure that uses the stain carbolfuchsin and a
mordant to thicken the diameters of the flagella so they can be seen through a light microscope.
Hybridization Correct Ans-The process of taking a single strand of DNA from one species to
test its ability to bind (hybridize) with the DNA strand from another species; the stronger the
degree of binding, the greater the similarity between the two species Combining of 2 varieties or
species of organisms to create a hybrid
Immunofluorescence Correct Ans-A technique for the identification of an antigen by
exposing it to known antibodies. A fluorochrome is added that chemically combines with the
antibodies; as the fluorescent antibodies bind to the antigens on the bacterial surface, the bacteria
fluoresce.
MacConkey Agar Correct Ans-Selective and differential medium that is a combination of bile
salts, lactose, and crystal violet. The agar differentiates between lactose-fermenting and non-
lactose fermenting bacteria.
metric system Correct Ans-The decimal measuring system based on the meter, liter, and gram
as units of length, capacity, and weight or mass.
mordant Correct Ans-a chemical additive added to a stain to increase the effectiveness of the
stain
Complete Solutions
Culture Correct Ans-A population of microbes on a prepared culture medium conductive to
their growth
Culture media Correct Ans-A prepared substance which contains nutrients to grow microbes.
Types of culture media include broths, gelatins, and agar and may be placed in Petri dishes or
test tubes.
differential media Correct Ans-Types of media used to distinguish particular colonies of
bacteria from other colonies that are growing on the same Petri dish.
enriched media Correct Ans-Either a solid or broth mixture that contains nutrients which
promote the growth of fastidious microbes.
Enzyme Correct Ans-A complex protein with the ability to induce a chemical change with out
being changed itself. When secreted by a bacterium, it invaded the hosts cells and tissues to
produce a reaction including breakdown of substances into simple compounds or causing disease
enzyme-linked immunosorbent assay (ELISA) test Correct Ans-A laboratory test using
antisera to identify a particular bacteria, antibodies, or hormones
, fastidius bacteria Correct Ans-Bacteria which have complex and specific nutritional
requirements for growth and survival
flagella staining Correct Ans-Staining procedure that uses the stain carbolfuchsin and a
mordant to thicken the diameters of the flagella so they can be seen through a light microscope.
Hybridization Correct Ans-The process of taking a single strand of DNA from one species to
test its ability to bind (hybridize) with the DNA strand from another species; the stronger the
degree of binding, the greater the similarity between the two species Combining of 2 varieties or
species of organisms to create a hybrid
Immunofluorescence Correct Ans-A technique for the identification of an antigen by
exposing it to known antibodies. A fluorochrome is added that chemically combines with the
antibodies; as the fluorescent antibodies bind to the antigens on the bacterial surface, the bacteria
fluoresce.
MacConkey Agar Correct Ans-Selective and differential medium that is a combination of bile
salts, lactose, and crystal violet. The agar differentiates between lactose-fermenting and non-
lactose fermenting bacteria.
metric system Correct Ans-The decimal measuring system based on the meter, liter, and gram
as units of length, capacity, and weight or mass.
mordant Correct Ans-a chemical additive added to a stain to increase the effectiveness of the
stain
