Lab 7 Practical Questions With Complete
Solutions
Central Dogma DNA --> RNA --> Proteins
mRNA Processing Eukaryotes ONLY, primary transcript is spliced in the nucleus
Transcription DNA --> RNA in the nucleus; mRNA contains exons only
Translation tRNA brings amino acids to corresponding mRNA codons in the ribosome
and builds a polypeptide chain
E-value Determines the probability that a match is due to chance (lower E-value means
it's a better match)
BLAST Program compares nucleotide or protein sequence to sequences in the database
and calculates statistical significance of matches. Proteins are easier to identify than DNA and
only require a 25% match whereas DNA requires a 70% match.
Solutions
Central Dogma DNA --> RNA --> Proteins
mRNA Processing Eukaryotes ONLY, primary transcript is spliced in the nucleus
Transcription DNA --> RNA in the nucleus; mRNA contains exons only
Translation tRNA brings amino acids to corresponding mRNA codons in the ribosome
and builds a polypeptide chain
E-value Determines the probability that a match is due to chance (lower E-value means
it's a better match)
BLAST Program compares nucleotide or protein sequence to sequences in the database
and calculates statistical significance of matches. Proteins are easier to identify than DNA and
only require a 25% match whereas DNA requires a 70% match.
