Lab 8 Practical Questions With Complete Solutions

Lab 8 Practical Questions With Complete
Solutions

Attenuated E. Coli Loss ability to cause disease




DNA Isolation First step in many procedures such as; genetic screening, paternity tests,

forensics, molecule cloning.




Vortex Used to rapidly mix contents of test tube




Minifuge Used to quickly spin with no control over speed or time




Table-top Microcntrifuge Spins tubes at programmable speeds & times




Materials for DNA Isolation Lysis buffer


Potassium acetate

Isopropanol

Ethanol

, Lab 8 Practical Questions With Complete
Solutions
Lysis Buffer Involved in DNA isolation; Breaks open bacteria; contains TRIS HCL,

EDTA, & SDS




TRIS HCL Component of lysis buffer; gets the correct pH for DNA extraction & helps

permeabilize the membrane




EDTA Component of lysis buffer, "Chelating agent," inhibits DNAase enzyme (which

denatures DNA)




SDS Component of lysis buffer; detergent breaks down plasma membrane & releases

DNA from histones




Potassium Acetate Involved in DNA isolation; stabilizes DNA & allows for removal of

proteins




Isopropanol Involved in DNA isolation; precipitates genomic DNA. Isopropanol is

NONPOLAR (DNA is polar, so this is why it precipitates the DNA)