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QBM All of the Practice Exams with verified answers

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QBM All of the Practice Exams with verified answers
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a. protein as a ball-and-stick model, DNA as a molecular surface representation.
b. DNA as a ball-and-stick model, protein as a molecular surface representation.
c. both as ball-and-stick models.
d. protein as a cartoon model, DNA as a ball-and stick model.
e. both as cartoon models. Ans✓✓-d. protein as a cartoon model, DNA as a ball-
and stick model.


Oswald Avery showed that


a. proteins are encoded by RNA.
b. DNA is responsible for bacterial transformations.
c. protein is responsible for bacterial transformations.
d. point mutations are the cause of the polymorphisms observed by Griffith.
e. the ratio of As to Ts and Gs to Cs in a cell are equal. Ans✓✓-b. DNA is
responsible for bacterial transformations


Which of the following refers to manipulating a piece of DNA and putting it into a
plasmid for the purposes of gene studies, protein expression, and gene therapy?


a. Bioinformatics.
b. In silico research.
c. Recombinant DNA technology.

,d. Interdisciplinary research.
e. Gene Chip technology. Ans✓✓-c. Recombinant DNA technology.


Employers must inform employees and train them in the safe handling of
potentially harmful chemicals. This is required by


a. OSHA.
b. the MSDS.
c. the CDC.
d. individual employers who set their own standards.
e. The Laboratory Safety Officer, who enforces the rules and assesses monetary
fines for
those in violation. Ans✓✓-a. OSHA.


Research labs that handle pathogenic organisms for which there is no vaccine that
can cause diseases which are generally untreatable and fatal are assigned a rating
of


a. BSL-1 b. BSL-2 c. BSL-3 d. BSL-4 e. BSL-5 Ans✓✓-d. BSL-4


Where should you open bottles that contain volatile chemicals such as HCl or
SDS?
a. In the flammable cabinet.
b. In the sharps container.
c. In the chemical cabinet
d. In the cell culture hood.

,e. In the fume hood. Ans✓✓-e. In the fume hood.


Which type of gloves offer the least amount of protection from
chemical/biological hazards?
a. Plastic.
b. Latex.
c. Chloroprene.
d. Nitrile. Ans✓✓-a. Plastic.


A device used to clean glassware and sterilize media using heat and pressure is
a(n)
a. sonicator.
b. ultrafiltrator.
c. autoclave.
d. fume hood.
e. biosafety hood. Ans✓✓-c. autoclave.


The least accurate way to transfer a specific volume would be with a


micropipette.
Mohr pipette.
volumetric pipette.
serological pipette.
Pasteur (transfer) pipette. Ans✓✓-Pasteur (transfer) pipette.

, A Mohr micropipette has a thinner tip, which is beneficial when using the pipette
to


A. transfer over 100 ml of sample in a single step.
B. work with viscous liquids.
C. dispense specific volumes several times from a single aspiration.
D. generate a standard curve when performing protein quantification. E. deliver
exactly 10 microliters. Ans✓✓-C. dispense specific volumes several times from a
single aspiration.


Some tips have cotton swabs in them to
a. increase the accuracy of pipetting by decreasing air vortices.
b. mark the maximum volume of the tip so you do not exceed it.
c. reduce pressure variations as you dispense the liquid.
d. prevent contamination of the pipettes and samples.
e. help the tip fit more snugly on the pipette. Ans✓✓-d. prevent contamination of
the pipettes and samples.


You have a 1.5 ml tube of frozen enzyme. Upon removing it from the freezer you
should thaw it and spin it down briefly in a centrifuge. What is the purpose of
this?
a. To remove any liquid from inside the cap.
b. To activate the enzyme.
c. To increase the sample's rate of thawing.
d. To reduce the surface tension so pipetting is more accurate. Ans✓✓-a. To
remove any liquid from inside the cap.

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