MBIO 3812 Exam 1 questions & correct
answers to pass
Glo Germ - correct answer ✔✔lotion containing small plastic that can only be seen under UV light
The most effective way of getting rid of germs - correct answer ✔✔handwashing
what needs to be provided to culture bacteria? - correct answer ✔✔nutrients such as carbon nitrogen,
amino acids, lipids, and carbohydrates
What are the two media types? - correct answer ✔✔broth or agar
undefined media - correct answer ✔✔the exact ingredients and their amounts are not known
defined media - correct answer ✔✔all ingredients & amounts are known
Agar originates from... - correct answer ✔✔seaweed.
Autoclaving - correct answer ✔✔- Method of sterilization using steam under pressure
- the most effective sterilization method
Autoclave parameters - correct answer ✔✔temperature, time, and steam pressure
How you can tell an item is sterile after its been autoclaved? - correct answer ✔✔The tape will have
black stripes
when done dispensing TSB into appropriate test tubes, where should the remaining solution be
discarded? - correct answer ✔✔down the sink
,Once plates have solidified, do you label each plate or stack ? - correct answer ✔✔Each stack
Why are aseptic techniques important? - correct answer ✔✔they help to prevent other microorganisms
from the air and surfaces from contaminating the microorganisms being prepared.
Tools used during aseptic transfer - correct answer ✔✔inoculating loop and needle, transfer pipette, &
glass cell spreader
when inoculating an agar slant, make _____ pattern. - correct answer ✔✔zig zag
Goal of isolation - correct answer ✔✔to obtain a pure culture from a mixed culture
During a quadrant streak, the concentration... - correct answer ✔✔decreases with each quadrant
quadrant streak - correct answer ✔✔aseptically removing a sample from mixed culture w/ inoculating
loop to obtain isolated colonies
During a quadrant streak, pick up the plate: - correct answer ✔✔lid side down
ubiquity - correct answer ✔✔existing everywhere
types of microorganisms - correct answer ✔✔pathogenic, opportunistic & free-living
why are streak environmental samples different from clinical samples? - correct answer ✔✔(1) bacteria
levels are likely to be lower in an environmental sample (2) when streaking in the environment, there is
usually no flame source
(3) Isolation streaking in the environment likely wouldn't be well separated
What does an environmental streak look like? - correct answer ✔✔Zig zag pattern
, What step shouldn't be done in a quadrant streak? - correct answer ✔✔transfer bacteria from broth
before each streak
T/F: opportunistic pathogens always cause disease - correct answer ✔✔false
When labeling plates, what info should be included? - correct answer ✔✔initials, date, section #, media
type, organism used
Importance of colony morphology - correct answer ✔✔can give you an idea of how many different
bacterial species are in a sample
distinguishing features of colonies: - correct answer ✔✔form, texture, margin, growth patterns,
elevation, size
Steps for making spread plates: - correct answer ✔✔1)Label plates according to tubes; place 0.1 mL of
bacterial dilution onto the MIDDLE of the agar plate
2)Put cell spreader into jar of alcohol, let excess alcohol drip off
3)Pass cell spreader over a lit bunsen burner
4)Cool cell spreader by touching it to agar dish AWAY from bacterial solution; spread sample all over the
surface of the plate
5)Allow solution to be absorbed into the agar; turn plate over and incubate overnight
T/F: 1 colony = 1 bacterial cell - correct answer ✔✔False
Colony - correct answer ✔✔one bacterial cell that has divided into a group of identical cells
Determining CFU/mL - correct answer ✔✔(# of colonies)/(DF)(VP)
TNTC - correct answer ✔✔too numerous to count
answers to pass
Glo Germ - correct answer ✔✔lotion containing small plastic that can only be seen under UV light
The most effective way of getting rid of germs - correct answer ✔✔handwashing
what needs to be provided to culture bacteria? - correct answer ✔✔nutrients such as carbon nitrogen,
amino acids, lipids, and carbohydrates
What are the two media types? - correct answer ✔✔broth or agar
undefined media - correct answer ✔✔the exact ingredients and their amounts are not known
defined media - correct answer ✔✔all ingredients & amounts are known
Agar originates from... - correct answer ✔✔seaweed.
Autoclaving - correct answer ✔✔- Method of sterilization using steam under pressure
- the most effective sterilization method
Autoclave parameters - correct answer ✔✔temperature, time, and steam pressure
How you can tell an item is sterile after its been autoclaved? - correct answer ✔✔The tape will have
black stripes
when done dispensing TSB into appropriate test tubes, where should the remaining solution be
discarded? - correct answer ✔✔down the sink
,Once plates have solidified, do you label each plate or stack ? - correct answer ✔✔Each stack
Why are aseptic techniques important? - correct answer ✔✔they help to prevent other microorganisms
from the air and surfaces from contaminating the microorganisms being prepared.
Tools used during aseptic transfer - correct answer ✔✔inoculating loop and needle, transfer pipette, &
glass cell spreader
when inoculating an agar slant, make _____ pattern. - correct answer ✔✔zig zag
Goal of isolation - correct answer ✔✔to obtain a pure culture from a mixed culture
During a quadrant streak, the concentration... - correct answer ✔✔decreases with each quadrant
quadrant streak - correct answer ✔✔aseptically removing a sample from mixed culture w/ inoculating
loop to obtain isolated colonies
During a quadrant streak, pick up the plate: - correct answer ✔✔lid side down
ubiquity - correct answer ✔✔existing everywhere
types of microorganisms - correct answer ✔✔pathogenic, opportunistic & free-living
why are streak environmental samples different from clinical samples? - correct answer ✔✔(1) bacteria
levels are likely to be lower in an environmental sample (2) when streaking in the environment, there is
usually no flame source
(3) Isolation streaking in the environment likely wouldn't be well separated
What does an environmental streak look like? - correct answer ✔✔Zig zag pattern
, What step shouldn't be done in a quadrant streak? - correct answer ✔✔transfer bacteria from broth
before each streak
T/F: opportunistic pathogens always cause disease - correct answer ✔✔false
When labeling plates, what info should be included? - correct answer ✔✔initials, date, section #, media
type, organism used
Importance of colony morphology - correct answer ✔✔can give you an idea of how many different
bacterial species are in a sample
distinguishing features of colonies: - correct answer ✔✔form, texture, margin, growth patterns,
elevation, size
Steps for making spread plates: - correct answer ✔✔1)Label plates according to tubes; place 0.1 mL of
bacterial dilution onto the MIDDLE of the agar plate
2)Put cell spreader into jar of alcohol, let excess alcohol drip off
3)Pass cell spreader over a lit bunsen burner
4)Cool cell spreader by touching it to agar dish AWAY from bacterial solution; spread sample all over the
surface of the plate
5)Allow solution to be absorbed into the agar; turn plate over and incubate overnight
T/F: 1 colony = 1 bacterial cell - correct answer ✔✔False
Colony - correct answer ✔✔one bacterial cell that has divided into a group of identical cells
Determining CFU/mL - correct answer ✔✔(# of colonies)/(DF)(VP)
TNTC - correct answer ✔✔too numerous to count
