BBK Practical Final Exam QUESTIONS AND CORRECT
ANSWERS 2024-2025 LATEST VERSION//GRADED A+
B - ANSWER-Which of the following situations can enhance hemagglutination reactions?
a. testing at a temperature higher than 37°C
b. increasing the incubation time
c. increasing the antigen concentration d. making the pH greater than 7
A - ANSWER-What term describes molecules that bind to an antigen to increase phagocytosis?
a. opsonins
b. cytokines
c. haptens
d. isotypes
C - ANSWER-After performing a tube test, the supernatant of the test was pinkish and the red
cellbutton was small. How do you interpret the result of this test?
a. false-positive
b. false-negative
c. positive
d. negative
A - ANSWER-How would you grade an agglutination reaction if you observe many small
agglutinates in a background of free cells in tube testing?
a. 1+
b. 2+
c. 3+
d. 4+
A - ANSWER-To determine the presence of a red cell antibody in a patient's sample, what is the
source of antigen?
a. commercial reagent red cells
,b. commercial antisera
c. patient serum
d. patient red cells
E - ANSWER-How does complement activation demonstrate within the body?
a. cell lysis
b. enhanced cell clearance
c. neutrophil activation
d. generation of vasoactive amines
e. all of the above
B - ANSWER-After addition of anti-A reagent to a patient's red cell suspension, agglutination
wasobserved. The result with anti-B reagent was negative. What is the interpretation of this patient's
ABO typing?
a. patient is group B
b. patient is group A
c. cannot interpret this test
d. false-positive result
Genotype - ANSWER-the actual genes inherited from each parent
Phenotype - ANSWER-the physical expression of inherited traits
made form several different clones of B cells that secrete antibodies of different specificities -
ANSWER-All of the following are true of monoclonal antibody reagents except:
*products of a single clone of B cells
*recognize one specific epitope of the antigen
*increased the accuracy and cost of blood typing reagents by using murine antibodies instead of
human donors.
*made form several different clones of B cells that secrete antibodies of different specificities
, Antiglobulin reagents - ANSWER-anti-IgG or anti- C3d or a combination of anti-IgG and anti-C3d
Antisera - ANSWER-known red cell antibodies
Reagent red cells - ANSWER-known red cell antigens
Potentiators - ANSWER-used to enhance antibodies
True - ANSWER-The major advantages of solid phase technology include standardization and
stable, defined endpoints, leading to more objective and consistent interpretations of agglutination
reactions.
True
False
True - ANSWER-Anti A and anti B commercial reagent are used to determine whether an
individual's red cells contain possess the A and B antigens of the ABO blood group system.
True
False
50% A and 50% O - ANSWER-Based on the Punnett Square, what are the probabilities of a child's
blood type if the mother is AO and the father is OO?
True - ANSWER-Except in very rare circumstances, all blood bank reagents must not be used past
the expiration date and only used according to the directions in the package insert.
True
False
they are plant extracts useful as blood banking reagents - ANSWER-Which of the following is true
regarding lectins?
*they are antibodies
*they are plant extracts useful as blood banking reagents
ANSWERS 2024-2025 LATEST VERSION//GRADED A+
B - ANSWER-Which of the following situations can enhance hemagglutination reactions?
a. testing at a temperature higher than 37°C
b. increasing the incubation time
c. increasing the antigen concentration d. making the pH greater than 7
A - ANSWER-What term describes molecules that bind to an antigen to increase phagocytosis?
a. opsonins
b. cytokines
c. haptens
d. isotypes
C - ANSWER-After performing a tube test, the supernatant of the test was pinkish and the red
cellbutton was small. How do you interpret the result of this test?
a. false-positive
b. false-negative
c. positive
d. negative
A - ANSWER-How would you grade an agglutination reaction if you observe many small
agglutinates in a background of free cells in tube testing?
a. 1+
b. 2+
c. 3+
d. 4+
A - ANSWER-To determine the presence of a red cell antibody in a patient's sample, what is the
source of antigen?
a. commercial reagent red cells
,b. commercial antisera
c. patient serum
d. patient red cells
E - ANSWER-How does complement activation demonstrate within the body?
a. cell lysis
b. enhanced cell clearance
c. neutrophil activation
d. generation of vasoactive amines
e. all of the above
B - ANSWER-After addition of anti-A reagent to a patient's red cell suspension, agglutination
wasobserved. The result with anti-B reagent was negative. What is the interpretation of this patient's
ABO typing?
a. patient is group B
b. patient is group A
c. cannot interpret this test
d. false-positive result
Genotype - ANSWER-the actual genes inherited from each parent
Phenotype - ANSWER-the physical expression of inherited traits
made form several different clones of B cells that secrete antibodies of different specificities -
ANSWER-All of the following are true of monoclonal antibody reagents except:
*products of a single clone of B cells
*recognize one specific epitope of the antigen
*increased the accuracy and cost of blood typing reagents by using murine antibodies instead of
human donors.
*made form several different clones of B cells that secrete antibodies of different specificities
, Antiglobulin reagents - ANSWER-anti-IgG or anti- C3d or a combination of anti-IgG and anti-C3d
Antisera - ANSWER-known red cell antibodies
Reagent red cells - ANSWER-known red cell antigens
Potentiators - ANSWER-used to enhance antibodies
True - ANSWER-The major advantages of solid phase technology include standardization and
stable, defined endpoints, leading to more objective and consistent interpretations of agglutination
reactions.
True
False
True - ANSWER-Anti A and anti B commercial reagent are used to determine whether an
individual's red cells contain possess the A and B antigens of the ABO blood group system.
True
False
50% A and 50% O - ANSWER-Based on the Punnett Square, what are the probabilities of a child's
blood type if the mother is AO and the father is OO?
True - ANSWER-Except in very rare circumstances, all blood bank reagents must not be used past
the expiration date and only used according to the directions in the package insert.
True
False
they are plant extracts useful as blood banking reagents - ANSWER-Which of the following is true
regarding lectins?
*they are antibodies
*they are plant extracts useful as blood banking reagents
