MLT ASCP Chemistry Exam | All Questions and
Answers | Graded A+ | Latest Edition
What does denaturation of proteins refer to? - ANSWER -alteration in tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANSWER -
cystinuria
How many immunoglobulin classes are currently recognized? - ANSWER -five
which reagent is employed in the serum protein determination? - ANSWER -
biuret
What should hemolyzed serum not be used for total protein analysis? - ANSWER
-hemoglobin is absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - ANSWER -is relatively free from
interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding
techniques? - ANSWER -there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWER -
albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - ANSWER -acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10%
beta-globulin, and 45% gamma-globulin, then what condition may exist in the
patient? - ANSWER -monoclonal gammopathy
,Glycoproteins and mucoproteins are usually bound to which substance? -
ANSWER -carbohydrates
Name some glycoproteins? - ANSWER -ceruloplasmin, follicle-stimulating
hormone, cryoglobulin
What applies to cryoglobulins? - ANSWER -they are temperature-sensitive
proteins
What is true of albumen? - ANSWER -it is produced in the liver and it is water
soluble
Decreased alpha-fetoprotein values are associated with what condition? -
ANSWER -Down Syndrome
What protein transports iron? - ANSWER -transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - ANSWER -
blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? -
ANSWER -ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxime method? - ANSWER -The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? -
ANSWER -elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - ANSWER -Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - ANSWER -titan yellow
, The creatinine clearance test is based on what assumption? - ANSWER -
Creatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration
rate? - ANSWER -creatinine
Creatinine clearance formula - ANSWER -UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL;
serum creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the
clearance, assuming average body surface area? - ANSWER -64 mL/minute
What disease state is associated with an elevation of serum uric acid? - ANSWER
-gout
Which reagent is employed in the alkaline oxidation of uric acid? - ANSWER -
phosphotungistic acid
What does the uricase method for uric acid depend on? - ANSWER -ultraviolet
absorption at 290 nm before/after treatment with uricase
Which analytes is used as a prognostic indicator for liver failure? - ANSWER -
ammonia
Which disaccharide is composed of two glucose molecules? - ANSWER -maltose
What term is used to describe the process of glycogen degradation? - ANSWER -
glycogenolysis
What is the renal threshold for glucose? - ANSWER -140-160 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? -
ANSWER -insulin
Answers | Graded A+ | Latest Edition
What does denaturation of proteins refer to? - ANSWER -alteration in tertiary
structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANSWER -
cystinuria
How many immunoglobulin classes are currently recognized? - ANSWER -five
which reagent is employed in the serum protein determination? - ANSWER -
biuret
What should hemolyzed serum not be used for total protein analysis? - ANSWER
-hemoglobin is absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - ANSWER -is relatively free from
interference by lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding
techniques? - ANSWER -there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWER -
albumin
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - ANSWER -acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10%
beta-globulin, and 45% gamma-globulin, then what condition may exist in the
patient? - ANSWER -monoclonal gammopathy
,Glycoproteins and mucoproteins are usually bound to which substance? -
ANSWER -carbohydrates
Name some glycoproteins? - ANSWER -ceruloplasmin, follicle-stimulating
hormone, cryoglobulin
What applies to cryoglobulins? - ANSWER -they are temperature-sensitive
proteins
What is true of albumen? - ANSWER -it is produced in the liver and it is water
soluble
Decreased alpha-fetoprotein values are associated with what condition? -
ANSWER -Down Syndrome
What protein transports iron? - ANSWER -transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - ANSWER -
blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? -
ANSWER -ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxime method? - ANSWER -The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? -
ANSWER -elevated blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - ANSWER -Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - ANSWER -titan yellow
, The creatinine clearance test is based on what assumption? - ANSWER -
Creatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration
rate? - ANSWER -creatinine
Creatinine clearance formula - ANSWER -UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL;
serum creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the
clearance, assuming average body surface area? - ANSWER -64 mL/minute
What disease state is associated with an elevation of serum uric acid? - ANSWER
-gout
Which reagent is employed in the alkaline oxidation of uric acid? - ANSWER -
phosphotungistic acid
What does the uricase method for uric acid depend on? - ANSWER -ultraviolet
absorption at 290 nm before/after treatment with uricase
Which analytes is used as a prognostic indicator for liver failure? - ANSWER -
ammonia
Which disaccharide is composed of two glucose molecules? - ANSWER -maltose
What term is used to describe the process of glycogen degradation? - ANSWER -
glycogenolysis
What is the renal threshold for glucose? - ANSWER -140-160 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? -
ANSWER -insulin
