Molecular Biology - Molecular Techniques UPDATED ACTUAL Questions and CORRECT Answers

Molecular Biology - Molecular Techniques
UPDATED ACTUAL Questions and
CORRECT Answers
The resolution of DNA on an agarose gel - CORRECT ANSWER - Smaller size of DNA
can be resolved as the concentration of agarose increases


Detect RFLP (Restiction fragment length polymorphism) - CORRECT ANSWER -
Southern blot


Reverse dot blot - CORRECT ANSWER - Attaching multiple probes to nitrocellulose
membrane and then hybridizing a single labeled patient sample to multiple probes


The highest stringency for washing a Southern blot after hybridization - CORRECT
ANSWER - High temperature, low salt concentration



The use of the Klenow fragment of E.coli DNA polymerase I - CORRECT ANSWER - To
label DNA probes by random primer labeling


A solution hybridization method for the detection of nucleic acid:protein interactions -
CORRECT ANSWER - Gel mobility shift assay



The probe used to detect trisomy 21 by interphase FISH - CORRECT ANSWER - Alpha
satellite DNA probe


Ethanol volumes added to 1 volume of a DNA:salt solution to case DNA precipitate -
CORRECT ANSWER -2



Chelex 100 resom - CORRECT ANSWER - Extract DNA from cells