Revision Examination Tests
“Come all for this Greatness”
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MICROBIOLOGY LAB QUIZ 1 2024-2025 ACTUAL EXAM COMPLETE 200-
QUESTIONS AND CORRECT DETAILED ANSWERS
Quiz Proper use of the microscope
Ans: all specimens must be viewed under cover glass, focus with scanning
objectives first, use course adjustment knob, once in focus, use the fine focusing
knob (when viewing with low or high-power objectives)
Quiz Depth of Field
Ans: refers to the thickness of the plane of focus
Quiz Field of View
Ans: area that is visible when using the microscope
Quiz Why use a microscope?
Ans: To view and study the microscopic world of organisms and objects less than
5mm in size that cohabitate with us on our globe.
Quiz Ocular lens (location and job)
Ans: Area you place your eyes to see in scope (top of the tube) - magnifies the
image formed by the objective lens
Quiz Objective lenses (location and job)
Ans: the lenses right above where the sample is placed to be viewed. Can be
changed to different magnifications (scanning, low, high). - magnifies the object
Quiz Specimen holder (location and job)
Ans: located right under the objective lenses - holds specimen in place
Quiz Mechanical Stage (location and job)
, Ans: platform that you place your slides on - holds them in place with help of
specimen holders and can be be moved up and down or left to right
Quiz Light intensity control (location and job)
Ans: usually by or with the main power switch. Controls the intensity of the light
Quiz x-y axis control knob (location and job)
Ans: attached to the stage (mechanical) - moves the stage along the x/y axis. One
knob for back/front, one knob for left/right
Quiz Course focusing knob (location and job)
Ans: located on side of microscope usually with fine focus knob - rough focus knob
only used with high power objective
Quiz Fine focusing knob (location and job)
Ans: located on side of microscope usually with coarse focus knob - focus knob
used to fine tune the focus on the specimen
Quiz Iris diaphragm (location and job)
Ans: under the condenser and above the light source - controls the amount of light
that enters the condenser.
Quiz Condenser (location and job)
Ans: lens mounted below the stage and above iris diaphram - used to focus the light
on a small area above the stage
Quiz Light source (location and job)
Ans: mounted under the condenser (maybe in/under the base) - the light source for
the microscope
Quiz Base (location and job)
Ans: located at bottom of microscope - the bottom support of microscope
Quiz How to you lift a microscope?
Ans: Pick it up by having one hand under the base and one hand behind the
microscope
Quiz What does par focal mean?