SERIES MICROBIOLOGY LAB EXAM 2 COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

Question: gram-negative Ans: appear pink Question: Isolation by Dilution Ans: -reducing number of bacteria so that individual colonies may be isolated -streak plate technique is commonly used to separate mixed cultures to isolate colonies that can be used to make a pure culture Question: Selective media Ans: promotes the growth of some organisms and inhibits the growth of others Question: Differential media Ans: show different growth characteristics for certain organisms, such as a color change Question: TSA SERIES Ans: Tryptic Soy Agar -neither selective nor differential Question: MSA Ans: Mannitol Salt Agar -both selective and differential -selects for salt tolerant (halophiles) -differential- mannitol fermenters change media from red to yellow Question: EMB Ans: Eosin Methylene Blue Agar -both selective and differential -selects for gram-negative and inhibits gram-positive -differential- lactose fermenters exhibit metallic green sheen Question: Carbohydrate Catabolism labs Ans: Starch hydrolysis and OF glucose deeps Question: Starch hydrolysis SERIES Ans: -differential test -if starch is no longer in media, the bacteria will test positive for amylase -Flood agar with iodine; iodine will turn media purple if it contains starch -positive result will have zone of clearing and no color change in media -negative result will have no zone of clearing and color change of media to dark purple Question: OF glucose de