Microbiology Lab Practical 1 Study
guide
Illuminator Correct Ans-Filter for shorter λ
Condenser microscope Correct Ans-Focuses light into specimen
Diaphragm (iris) Correct Ans-Controls amount of light to specimen
Objective lenses at nosepiece Correct Ans-Magnifies image 10, 40, or 100 times
Ocular lens Correct Ans-magnifies image 10X
Course adjustment Correct Ans-focuses specimen in a broad way
Fine adjustment Correct Ans-fine focuses specimen
Stage Correct Ans-a platform used to hold the slide containing the specimen
Arm Correct Ans-serves as a handle for carrying microscope
Mechanical stage adjuster Correct Ans-moves stage forward and back
, Stage clip Correct Ans-Holds microscope slide in position
Magnification Correct Ans-increase in apparent size
Resolution Correct Ans-clarity
Oil has the __________ refractive index as glass Correct Ans-same
This is the part of the microscope that should not be
touched under high power. Correct Ans-the course adjustment knob
How does the light intensity change when a student goes from a low power objective to a high
power objective? Correct Ans-The field of vision narrowed
Which lens do you use oil with? Correct Ans-100X lens
How does immersion oil work to enhance resolution? Correct Ans-Same refractive index as
glass; prevents loss of light to air
What is the maximum resolution of your microscope (in micrometers) or how far apart do two
organisms have to be from one another in order to see them as 2 different organisms under the
scope? Correct Ans-.2 um with 100X
guide
Illuminator Correct Ans-Filter for shorter λ
Condenser microscope Correct Ans-Focuses light into specimen
Diaphragm (iris) Correct Ans-Controls amount of light to specimen
Objective lenses at nosepiece Correct Ans-Magnifies image 10, 40, or 100 times
Ocular lens Correct Ans-magnifies image 10X
Course adjustment Correct Ans-focuses specimen in a broad way
Fine adjustment Correct Ans-fine focuses specimen
Stage Correct Ans-a platform used to hold the slide containing the specimen
Arm Correct Ans-serves as a handle for carrying microscope
Mechanical stage adjuster Correct Ans-moves stage forward and back
, Stage clip Correct Ans-Holds microscope slide in position
Magnification Correct Ans-increase in apparent size
Resolution Correct Ans-clarity
Oil has the __________ refractive index as glass Correct Ans-same
This is the part of the microscope that should not be
touched under high power. Correct Ans-the course adjustment knob
How does the light intensity change when a student goes from a low power objective to a high
power objective? Correct Ans-The field of vision narrowed
Which lens do you use oil with? Correct Ans-100X lens
How does immersion oil work to enhance resolution? Correct Ans-Same refractive index as
glass; prevents loss of light to air
What is the maximum resolution of your microscope (in micrometers) or how far apart do two
organisms have to be from one another in order to see them as 2 different organisms under the
scope? Correct Ans-.2 um with 100X
