Microbiology lab practical exam 1 with Answers

Microbiology lab practical exam 1 with
Answers
Wet mount steps: Correct Ans-1.water

2. specimen

3. Cover slip over specimen



hanging drop steps: Correct Ans-1. Vaseline around cover slip

2. micro organism in center of cover slip

3. place concave slide on cover slip



Gram stain steps: Correct Ans-1. Crystal Violet

2. Gram's Iodine

3. Ethyl Alcohol

4. Safranin Red



negative stain steps: Correct Ans-1. nigrosin

2.heat loop

3.bacteria

4. slide to spread mixture

5. air dry

, acid-fast stain steps: Correct Ans-1. carbolfuchsin

2. acid alcohol

3. methylene blue

4. wash & dry



simple stain steps Correct Ans-1. fixed smear slide

2. dyes

3. rinse & dry



nutrient broth Correct Ans--liquid media used to grow bacteria




agar Correct Ans--used to culture microorganisms




candle jar Correct Ans--used for anaerobic organisms




Petri dish Correct Ans--Used to culture or observe bacteria growth




slant Correct Ans--a medium for which bacteria can grow on.




E.coli Gram stain Correct Ans-red colored rods




S. epidermidis Gram stain Correct Ans-purple colored grapes