microbiology lab final exam with
Complete Solutions
Which staining technique is used to determine the thickness of the peptidoglycan layer based on
the cell wall composition? Correct Ans-Gram stain
Where would you properly dispose of the following items? Correct Ans-Contaminated slides:
Slide trays on student benches
Pipettes: Nalgene buckets on student benches
Used test tubes: Trash cart at front of lab
Uncontaminated broken glass: Broken glass box at front of lab (and notify the TA!)
When are open toed-shoes and shorts allowed in the microbiology labs? Correct Ans-Never
What do you use to clean the ocular lenses and objective lenses on your microscope? Correct
Ans-Lens cleaner and lens paper
________________________ microscopy is used to view dead, stained microorganisms, and
________________________ microscopy is used to view live, unstained microorganisms
Correct Ans-Brightfield, phase-contrast
What is the purpose of isolation streaks on your environmental isolate? Correct Ans-To purify
one species of bacteria
, In microscopy, what are the three main factors to obtain a good image? Correct Ans-
Magnification, resolution, and contrast
Which objective lens is used with immersion oil? Correct Ans-100X objective lens
When you first enter the lab and again before you leave the lab, what are two things that you
should always do? Correct Ans-Wash your hands and wipe down the lab bench with QT
solution
In Exercise 1 Part 2 Phase-Contrast Microscopy, ______________ ______________ was used to
visualize bacteria at 1000x total magnification with oil immersion. Correct Ans-Bacillus
megaterium
Which of the following is a growth streak? Correct Ans-perfect up down streak
______________ ______________ is the positive control organism for the capsule stain.
Correct Ans-Klebsiella pneumoniae
With regard to staining techniques used in this lab, what is the definition of a control organism?
Correct Ans-A control organism is known to have a positive or negative result for a specific
staining technique and is used as a comparison to determine the staining result of an unknown
bacteria
Complete Solutions
Which staining technique is used to determine the thickness of the peptidoglycan layer based on
the cell wall composition? Correct Ans-Gram stain
Where would you properly dispose of the following items? Correct Ans-Contaminated slides:
Slide trays on student benches
Pipettes: Nalgene buckets on student benches
Used test tubes: Trash cart at front of lab
Uncontaminated broken glass: Broken glass box at front of lab (and notify the TA!)
When are open toed-shoes and shorts allowed in the microbiology labs? Correct Ans-Never
What do you use to clean the ocular lenses and objective lenses on your microscope? Correct
Ans-Lens cleaner and lens paper
________________________ microscopy is used to view dead, stained microorganisms, and
________________________ microscopy is used to view live, unstained microorganisms
Correct Ans-Brightfield, phase-contrast
What is the purpose of isolation streaks on your environmental isolate? Correct Ans-To purify
one species of bacteria
, In microscopy, what are the three main factors to obtain a good image? Correct Ans-
Magnification, resolution, and contrast
Which objective lens is used with immersion oil? Correct Ans-100X objective lens
When you first enter the lab and again before you leave the lab, what are two things that you
should always do? Correct Ans-Wash your hands and wipe down the lab bench with QT
solution
In Exercise 1 Part 2 Phase-Contrast Microscopy, ______________ ______________ was used to
visualize bacteria at 1000x total magnification with oil immersion. Correct Ans-Bacillus
megaterium
Which of the following is a growth streak? Correct Ans-perfect up down streak
______________ ______________ is the positive control organism for the capsule stain.
Correct Ans-Klebsiella pneumoniae
With regard to staining techniques used in this lab, what is the definition of a control organism?
Correct Ans-A control organism is known to have a positive or negative result for a specific
staining technique and is used as a comparison to determine the staining result of an unknown
bacteria
