Microbiology Lab Practical 1 Gacura-
Test Questions & Answers
Primary containment Correct Ans-protection of the lab personnel and the lab environment
Secondary Containment Correct Ans-protection of the outside lab environment from exposure
to infectious organisms
Disposal of biohazards Correct Ans-Place biohazard material in marked bag or container;
must be tagged or color coded
Disposal of Glass waste Correct Ans-can be washed and reused after autoclaving (Kills
station)
Disposal of plastic waste Correct Ans-Melt in autoclave to dispose after decontaminated
Office hours and location Correct Ans-Zurn 207 Monday/Wednesday 1-2:30
Cidal Correct Ans-killing
bactericidal
Static Correct Ans-Halts growth
, bacteriostatic
Resident Flora Correct Ans-microorganisms that normally reside on locations (like skin)
Hard to get rid of
transient flora Correct Ans-microbes that reside on the skin surface and are easily removed
(not typical residents on skin)- there from contamination
What type of agar do we use? Correct Ans-Tryptic Soy agar
Colony Correct Ans-visible cluster of microbes growing on solid media (1 cell asexually
reproduces until it's visible.
What temperature is an autoclave? Correct Ans-121 degrees Celsius
What temp did we incubate the hand washing agar lab dish? Correct Ans-37 degrees Celsius
(human body temp)
Inoculation loop Correct Ans-Used to transfer microbiological live specimens under sterile
conditions
Nutrient broth Correct Ans-liquid medium used to cultivate bacteria (it feeds bacteria)
Test Questions & Answers
Primary containment Correct Ans-protection of the lab personnel and the lab environment
Secondary Containment Correct Ans-protection of the outside lab environment from exposure
to infectious organisms
Disposal of biohazards Correct Ans-Place biohazard material in marked bag or container;
must be tagged or color coded
Disposal of Glass waste Correct Ans-can be washed and reused after autoclaving (Kills
station)
Disposal of plastic waste Correct Ans-Melt in autoclave to dispose after decontaminated
Office hours and location Correct Ans-Zurn 207 Monday/Wednesday 1-2:30
Cidal Correct Ans-killing
bactericidal
Static Correct Ans-Halts growth
, bacteriostatic
Resident Flora Correct Ans-microorganisms that normally reside on locations (like skin)
Hard to get rid of
transient flora Correct Ans-microbes that reside on the skin surface and are easily removed
(not typical residents on skin)- there from contamination
What type of agar do we use? Correct Ans-Tryptic Soy agar
Colony Correct Ans-visible cluster of microbes growing on solid media (1 cell asexually
reproduces until it's visible.
What temperature is an autoclave? Correct Ans-121 degrees Celsius
What temp did we incubate the hand washing agar lab dish? Correct Ans-37 degrees Celsius
(human body temp)
Inoculation loop Correct Ans-Used to transfer microbiological live specimens under sterile
conditions
Nutrient broth Correct Ans-liquid medium used to cultivate bacteria (it feeds bacteria)
