Microbiology Lab Quiz 1- Questions with
Correct Answers
Proper use of the microscope Correct Ans-all specimens must be viewed under cover glass,
focus with scanning objectives first, use course adjustment knob, once in focus, use the fine
focusing knob (when viewing with low or high power objectives)
Depth of Field Correct Ans-refers to the thickness of the plane of focus
Field of View Correct Ans-area that is visible when using the microscope
Why use a microscope? Correct Ans-To view and study the microscopic world of organisms
and objects less than 5mm in size that cohabitate with us on our globe.
Ocular lens (location and job) Correct Ans-Area you place your eyes to see in scope (top of
the tube) - magnifies the image formed by the objective lens
Objective lenses (location and job) Correct Ans-the lenses right above where the sample is
placed to be viewed. Can be changed to different magnifications (scanning, low, high). -
magnifies the object
Specimen holder (location and job) Correct Ans-located right under the objective lenses -
holds specimen in place
, Mechanical Stage (location and job) Correct Ans-platform that you place your slides on -
holds them in place with help of specimen holders and can be be moved up and down or left to
right
Light intensity control (location and job) Correct Ans-usually by or with the main power
switch. Controls the intensity of the light
x-y axis control knob (location and job) Correct Ans-attached to the stage (mechanical) -
moves the stage along the x/y axis. One knob for back/front, one knob for left/right
Course focusing knob (location and job) Correct Ans-located on side of microscope usually
with fine focus knob - rough focus knob only used with high power objective
Fine focusing knob (location and job) Correct Ans-located on side of microscope usually with
coarse focus knob - focus knob used to fine tune the focus on the specimen
Iris diaphragm (location and job) Correct Ans-under the condenser and above the light source
- controls the amount of light that enters the condenser.
Condenser (location and job) Correct Ans-lens mounted below the stage and above iris
diaphram - used to focus the light on a small area above the stage
Light source (location and job) Correct Ans-mounted under the condenser (maybe in/under
the base) - the light source for the microscope
Correct Answers
Proper use of the microscope Correct Ans-all specimens must be viewed under cover glass,
focus with scanning objectives first, use course adjustment knob, once in focus, use the fine
focusing knob (when viewing with low or high power objectives)
Depth of Field Correct Ans-refers to the thickness of the plane of focus
Field of View Correct Ans-area that is visible when using the microscope
Why use a microscope? Correct Ans-To view and study the microscopic world of organisms
and objects less than 5mm in size that cohabitate with us on our globe.
Ocular lens (location and job) Correct Ans-Area you place your eyes to see in scope (top of
the tube) - magnifies the image formed by the objective lens
Objective lenses (location and job) Correct Ans-the lenses right above where the sample is
placed to be viewed. Can be changed to different magnifications (scanning, low, high). -
magnifies the object
Specimen holder (location and job) Correct Ans-located right under the objective lenses -
holds specimen in place
, Mechanical Stage (location and job) Correct Ans-platform that you place your slides on -
holds them in place with help of specimen holders and can be be moved up and down or left to
right
Light intensity control (location and job) Correct Ans-usually by or with the main power
switch. Controls the intensity of the light
x-y axis control knob (location and job) Correct Ans-attached to the stage (mechanical) -
moves the stage along the x/y axis. One knob for back/front, one knob for left/right
Course focusing knob (location and job) Correct Ans-located on side of microscope usually
with fine focus knob - rough focus knob only used with high power objective
Fine focusing knob (location and job) Correct Ans-located on side of microscope usually with
coarse focus knob - focus knob used to fine tune the focus on the specimen
Iris diaphragm (location and job) Correct Ans-under the condenser and above the light source
- controls the amount of light that enters the condenser.
Condenser (location and job) Correct Ans-lens mounted below the stage and above iris
diaphram - used to focus the light on a small area above the stage
Light source (location and job) Correct Ans-mounted under the condenser (maybe in/under
the base) - the light source for the microscope
