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Micro Lab Exam 2 transduction Questions
And Answers 100% Pass.
Why do we do plate-streaking? - Answer✔to create countable colonies, and identify them
What are the two materials used in an Ab susceptibility test, and how do you inoculate the plate?
- Answer✔alcohol wipes, and forceps; bacterial lawn
What two things does the zone of inhibition depend on? - Answer✔diffusion of the molecule,
and susceptibility
What are the effects the zone of inhibition has on bacteria? - Answer✔bactericidal or
bacteriostatic
What kind of test are enterotubes considered? - Answer✔biochemical tests/enzymes/color
changes
What does a color change in an enterotube signify? - Answer✔enzyme broke down whatever
was in the medium
What was a technique used in the enterotubes? - Answer✔aerobic holes
What do enterotubes identify? - Answer✔enteric gram negative bacilli
In a UV light test, specific damage is done to the bacteria with or without the lid? -
Answer✔without
How is bacteria damaged in UV light tests? - Answer✔damages DNA via thymine diamers
Which bacteria seemed to repair the UV damage better, and why? - Answer✔B. subtilis; E coli
isn't exposed to UV because its always in the intestine, whereas B subtilis lives in the soil, so its
adapted
When did you see the most damage? - Answer✔15-20 minutes
What makes electrophoresis sink? - Answer✔sucrose
What do the banding patterns in the electrophoresis indicate? - Answer✔common ancestry
What is used to cut DNA? - Answer✔restriction enzymes
Micro Lab Exam 2 transduction Questions
And Answers 100% Pass.
Why do we do plate-streaking? - Answer✔to create countable colonies, and identify them
What are the two materials used in an Ab susceptibility test, and how do you inoculate the plate?
- Answer✔alcohol wipes, and forceps; bacterial lawn
What two things does the zone of inhibition depend on? - Answer✔diffusion of the molecule,
and susceptibility
What are the effects the zone of inhibition has on bacteria? - Answer✔bactericidal or
bacteriostatic
What kind of test are enterotubes considered? - Answer✔biochemical tests/enzymes/color
changes
What does a color change in an enterotube signify? - Answer✔enzyme broke down whatever
was in the medium
What was a technique used in the enterotubes? - Answer✔aerobic holes
What do enterotubes identify? - Answer✔enteric gram negative bacilli
In a UV light test, specific damage is done to the bacteria with or without the lid? -
Answer✔without
How is bacteria damaged in UV light tests? - Answer✔damages DNA via thymine diamers
Which bacteria seemed to repair the UV damage better, and why? - Answer✔B. subtilis; E coli
isn't exposed to UV because its always in the intestine, whereas B subtilis lives in the soil, so its
adapted
When did you see the most damage? - Answer✔15-20 minutes
What makes electrophoresis sink? - Answer✔sucrose
What do the banding patterns in the electrophoresis indicate? - Answer✔common ancestry
What is used to cut DNA? - Answer✔restriction enzymes
