MB ASCP Test Well Answered Questions with
Correct Answers Graded A+, Download to Get
Perfect Score Key
260:230 should be... - Correct Answers-2.0, but lowers with contamination
A-DNA - Correct Answers-one of the possible double helical structures which DNA can adopt; right
handed double helix with shorter more compact helical structure than B-DNA form, slight increase in
number of base pairs per rotation (tighter rotation angle) and smaller rise/turn. (deepening of major
groove, shallowing of minor groove); found in dehydrated samples of DNA (crystallographic
experiments) dsRNA, DNA-RNA hybrid helices; 11 bp/turn
Allele specific PCR - Correct Answers-used to identify or utilize single nucleotide polymorphisms
(SNPs); distinguish between alleles; uses primers whose 3' ends encompass the SNP; will use 1 primer for
each allele and a 3rd primer in the opposite direction that works with both (2 forward and 1 reverse)
ALO1 recognizes - Correct Answers-AG|CT
amplicons - Correct Answers-amplification products
Annealing - Correct Answers-cooling to between 30-65 C (~5 less than melting temp of primer
template DNA duplex); allows primers to hybridize to complementary sites on target; excess primers
favor hybrid formation over reannealing of target strand
ASO - Correct Answers-allele specific oligonucleotide probes
B-form - Correct Answers-10.5 bp/turn; right handed helix; most common form
Base absorbance of A - Correct Answers-4.5
Base absorbance of C - Correct Answers-1.51
Base absorbance of G - Correct Answers-1.15
Base absorbance of T - Correct Answers-1.47 (DNA)
Base absorbance of U - Correct Answers-4 (RNA)
Benefits of Hot Start PCR - Correct Answers-increases specificity, reduces nonspecific bands,
prevents nonspecific extension or degradation of nucleic acid substrates at ambient temperatures
Benefits of Nested PCR - Correct Answers-increases specificity, reduces nonspecific bands (especially
longer products)
Benefits of Touchdown PCR - Correct Answers-increases specificity, reduces nonspecific bands
blot washing/development - Correct Answers-after hybridization block washed with NaCl and
detergent to remove excess probe
, Blotting - Correct Answers-Physical transfer of DNA from agarose gel to solid support (nylon
membrane) accomplished by capillary action, vacuum transfer, or electrotransfer; before blotting DNA
partially depurinated w/dilute HCl and denatured w/NaOH; attached to membrane by baking at ~80 C or
UV crosslinking
Branched DNA assay - Correct Answers-signal amplification assay used to detect nucleic acid
molecules; ssDNA molecules known as capture probe DNA molecules put into solid support, extender
DNA is added; target molecules then label extender, then pre-amplifier, then amplifier
Contamination - Correct Answers-check technique, check reagents, poor synthesis
Denaturation - Correct Answers-heat to near boiling to separate double stranded DNA into single
stranded (95 C)
DNA and RNA absorb uv light at what wave length - Correct Answers-260nm
DNA Ligase - Correct Answers-enzyme that facilitates joining of DNA strands together by catalyzing
formation of a phosphodiester bond, used for recombinant DNA
DNA Pol I - Correct Answers-4 enzymatic activities
1. 5' to 3' (forward) DNA dependent DNA polymerase activity, requires 3' primer site, template strand
2. 3' to 5' (reverse) exonuclease activity that mediates proofreading
3. 5' to 3' exonuclease activity mediating nick translation during DNA repair
4. 5' to 3' RNA dependent polymerase activity (lower efficiency and biological significance)
DNA Pol II - Correct Answers-Family B polymerase, 3' to 5' exonuclease activity, participates in
DNA repair, replication restart to bypass lesions, directs polymerase activity at the fork, help stalled Pol
III bypass terminal mismatches
DNA Pol III - Correct Answers-primary enzyme involved in E.Coli DNA replication
DNA Pol IV - Correct Answers-error prone DNA polymerase involved in non-targeted mutagenesis;
performs translesion synthesis @ stalled replication fork
DNA Pol V - Correct Answers-involved in SOS response and translesion synthesis
dNTPs - Correct Answers-incorporated by DNA polymerase activity, added via phosphodiester bonds
between 5' C of dNTP and OH group at 3' C of growing strand
Dot blot - Correct Answers-detects biomolecules; detect analyze, identify proteins, not separated by
electrophoresis, offers no info on size of molecules; can only confirm presence or absence of
biomolecules
Eastern blot - Correct Answers-analyzes post translational modifications like lipids, phospomoieties,
glycoconjugates
ECOR1 recognizes - Correct Answers-G|AATTC; leaves 5' overhang
Correct Answers Graded A+, Download to Get
Perfect Score Key
260:230 should be... - Correct Answers-2.0, but lowers with contamination
A-DNA - Correct Answers-one of the possible double helical structures which DNA can adopt; right
handed double helix with shorter more compact helical structure than B-DNA form, slight increase in
number of base pairs per rotation (tighter rotation angle) and smaller rise/turn. (deepening of major
groove, shallowing of minor groove); found in dehydrated samples of DNA (crystallographic
experiments) dsRNA, DNA-RNA hybrid helices; 11 bp/turn
Allele specific PCR - Correct Answers-used to identify or utilize single nucleotide polymorphisms
(SNPs); distinguish between alleles; uses primers whose 3' ends encompass the SNP; will use 1 primer for
each allele and a 3rd primer in the opposite direction that works with both (2 forward and 1 reverse)
ALO1 recognizes - Correct Answers-AG|CT
amplicons - Correct Answers-amplification products
Annealing - Correct Answers-cooling to between 30-65 C (~5 less than melting temp of primer
template DNA duplex); allows primers to hybridize to complementary sites on target; excess primers
favor hybrid formation over reannealing of target strand
ASO - Correct Answers-allele specific oligonucleotide probes
B-form - Correct Answers-10.5 bp/turn; right handed helix; most common form
Base absorbance of A - Correct Answers-4.5
Base absorbance of C - Correct Answers-1.51
Base absorbance of G - Correct Answers-1.15
Base absorbance of T - Correct Answers-1.47 (DNA)
Base absorbance of U - Correct Answers-4 (RNA)
Benefits of Hot Start PCR - Correct Answers-increases specificity, reduces nonspecific bands,
prevents nonspecific extension or degradation of nucleic acid substrates at ambient temperatures
Benefits of Nested PCR - Correct Answers-increases specificity, reduces nonspecific bands (especially
longer products)
Benefits of Touchdown PCR - Correct Answers-increases specificity, reduces nonspecific bands
blot washing/development - Correct Answers-after hybridization block washed with NaCl and
detergent to remove excess probe
, Blotting - Correct Answers-Physical transfer of DNA from agarose gel to solid support (nylon
membrane) accomplished by capillary action, vacuum transfer, or electrotransfer; before blotting DNA
partially depurinated w/dilute HCl and denatured w/NaOH; attached to membrane by baking at ~80 C or
UV crosslinking
Branched DNA assay - Correct Answers-signal amplification assay used to detect nucleic acid
molecules; ssDNA molecules known as capture probe DNA molecules put into solid support, extender
DNA is added; target molecules then label extender, then pre-amplifier, then amplifier
Contamination - Correct Answers-check technique, check reagents, poor synthesis
Denaturation - Correct Answers-heat to near boiling to separate double stranded DNA into single
stranded (95 C)
DNA and RNA absorb uv light at what wave length - Correct Answers-260nm
DNA Ligase - Correct Answers-enzyme that facilitates joining of DNA strands together by catalyzing
formation of a phosphodiester bond, used for recombinant DNA
DNA Pol I - Correct Answers-4 enzymatic activities
1. 5' to 3' (forward) DNA dependent DNA polymerase activity, requires 3' primer site, template strand
2. 3' to 5' (reverse) exonuclease activity that mediates proofreading
3. 5' to 3' exonuclease activity mediating nick translation during DNA repair
4. 5' to 3' RNA dependent polymerase activity (lower efficiency and biological significance)
DNA Pol II - Correct Answers-Family B polymerase, 3' to 5' exonuclease activity, participates in
DNA repair, replication restart to bypass lesions, directs polymerase activity at the fork, help stalled Pol
III bypass terminal mismatches
DNA Pol III - Correct Answers-primary enzyme involved in E.Coli DNA replication
DNA Pol IV - Correct Answers-error prone DNA polymerase involved in non-targeted mutagenesis;
performs translesion synthesis @ stalled replication fork
DNA Pol V - Correct Answers-involved in SOS response and translesion synthesis
dNTPs - Correct Answers-incorporated by DNA polymerase activity, added via phosphodiester bonds
between 5' C of dNTP and OH group at 3' C of growing strand
Dot blot - Correct Answers-detects biomolecules; detect analyze, identify proteins, not separated by
electrophoresis, offers no info on size of molecules; can only confirm presence or absence of
biomolecules
Eastern blot - Correct Answers-analyzes post translational modifications like lipids, phospomoieties,
glycoconjugates
ECOR1 recognizes - Correct Answers-G|AATTC; leaves 5' overhang
