1|Page
Microbiology Lab Exam 1 Note Cards Questions
and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification is - ✔✔1000x;10x ocular x 100X
objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass anddenature the proteins of
bacteria
name oneof the stains or regents used in the gram stain procedure - ✔✔gram crystal violet, gram iodine,
gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not contaminate what we are
working with as as to ensue that we do not acciently inoculate ouselves. example of aseptic techniques
are flaming the loop/needle sterilizing it, breifly flaming the test tube before and after obtaining the
sample.
the gram reaction is due to differences in the structure of wat part of the microbe - ✔✔its cell wall,
specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer compared to GN
bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure - ✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol fuschsin, methylen blue
name one of the stains in the endospore stain - ✔✔malachite green, safranin
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 2|Page
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast stain is to differentiate between
the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
what is the function of agarin the media - ✔✔agar allows media to remain solid at room temperature
what is the difference between broth and agar based media - ✔✔broth is liquid media at room
temperature, agar media is solid at room temp.
what is the funciton of media - ✔✔media is used to provide nutrients to a microbe as well as a growth
medium
what is the name of the plate inoculation technique - ✔✔quadrant steak plate
Why must the media we are making today be sterilized prior to us? - ✔✔the meda must be sterilized to
remove any bacterial contaminant that may be introduced when the media is made
how do you know yo have dont a successful streak plate? - ✔✔youve ended up with isolated colonies
what do you think will happen if you inoculated an obligate anerobe onto a TSA plate and incubated the
incubator we have been using? - ✔✔the obligate anerobe would not grow because it doesn't have the
ability to produce th necessary enzymes to dtoxify toxic oxygen species
What is one procedure used to determine oxygen requirement of a micrboe - ✔✔thioglycollate medium
or anaerobic bag
why are we working at differnt temperature in the growth curve excersize? - ✔✔to demonstrate th
differences in bacterial growth rate at different temperatures
why are we using the spectrophotometer in todarys class - ✔✔to determine the optical density of our
sample. this number will correlate the amount of bacteria in the sample
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
Microbiology Lab Exam 1 Note Cards Questions
and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification is - ✔✔1000x;10x ocular x 100X
objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass anddenature the proteins of
bacteria
name oneof the stains or regents used in the gram stain procedure - ✔✔gram crystal violet, gram iodine,
gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not contaminate what we are
working with as as to ensue that we do not acciently inoculate ouselves. example of aseptic techniques
are flaming the loop/needle sterilizing it, breifly flaming the test tube before and after obtaining the
sample.
the gram reaction is due to differences in the structure of wat part of the microbe - ✔✔its cell wall,
specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer compared to GN
bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure - ✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol fuschsin, methylen blue
name one of the stains in the endospore stain - ✔✔malachite green, safranin
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 2|Page
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast stain is to differentiate between
the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
what is the function of agarin the media - ✔✔agar allows media to remain solid at room temperature
what is the difference between broth and agar based media - ✔✔broth is liquid media at room
temperature, agar media is solid at room temp.
what is the funciton of media - ✔✔media is used to provide nutrients to a microbe as well as a growth
medium
what is the name of the plate inoculation technique - ✔✔quadrant steak plate
Why must the media we are making today be sterilized prior to us? - ✔✔the meda must be sterilized to
remove any bacterial contaminant that may be introduced when the media is made
how do you know yo have dont a successful streak plate? - ✔✔youve ended up with isolated colonies
what do you think will happen if you inoculated an obligate anerobe onto a TSA plate and incubated the
incubator we have been using? - ✔✔the obligate anerobe would not grow because it doesn't have the
ability to produce th necessary enzymes to dtoxify toxic oxygen species
What is one procedure used to determine oxygen requirement of a micrboe - ✔✔thioglycollate medium
or anaerobic bag
why are we working at differnt temperature in the growth curve excersize? - ✔✔to demonstrate th
differences in bacterial growth rate at different temperatures
why are we using the spectrophotometer in todarys class - ✔✔to determine the optical density of our
sample. this number will correlate the amount of bacteria in the sample
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
