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Microbiology Lab Final Exam Questions and Answers 100% Pass

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Microbiology Lab Final Exam Questions and Answers 100% Pass Give the steps of a PCR - Heat reaction to 95 C to denature DNA Cool rxn to 55 C to allow primers to anneal Heat rxn to 72 C to allow Taq polymerase to bind What machine is used to carry out a PCR reaction? - thermocycler How many cycles does a PCR rxn usually go? - 30 Name the 9 organisms used in the unknown lab - Bacillus subtillis Enterobacter aerogenes Entero cloacae Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa Salmonella typhimurium Staphylococcus aureus Staphylococcus epidermidis Which organisms can ferment lactose? - E. coli E. cloacae 2 | P a g e Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE E. aerogenes K. pneumonia Define caliform bacteria - rod-shaped, Gram-negative, non-spore forming, and motile or non-motile bacteria which can ferment lactose with the production of acid and gas Name the selective medias used in class - Blood Agar Bile Esculin Mannitol Salt Agar (MSA) MacConkey Agar (MaC) Eosin Methylene Blue (EMB) Hektoen Enteric Agar (HEA) Xylose Lysine Desoxiolate (XLD) Name the differential medias used in class - O/F Glucose

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Uploaded on: December 23, 2024
Number of pages: 8
Written in: 2024/2025
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microbiology lab final exam questions and answers

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Microbiology Lab Final Exam Questions and
Answers 100% Pass

Give the steps of a PCR - ✔✔Heat reaction to 95 C to denature DNA

Cool rxn to 55 C to allow primers to anneal

Heat rxn to 72 C to allow Taq polymerase to bind

What machine is used to carry out a PCR reaction? - ✔✔thermocycler

How many cycles does a PCR rxn usually go? - ✔✔30

Name the 9 organisms used in the unknown lab - ✔✔Bacillus subtillis

Enterobacter aerogenes

Entero cloacae

Escherichia coli

Klebsiella pneumoniae

Pseudomonas aeruginosa

Salmonella typhimurium

Staphylococcus aureus

Staphylococcus epidermidis

Which organisms can ferment lactose? - ✔✔E. coli

E. cloacae

Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE

, 2|Page

E. aerogenes

K. pneumonia

Define caliform bacteria - ✔✔rod-shaped, Gram-negative, non-spore forming, and motile or non-motile

bacteria which can ferment lactose with the production of acid and gas

Name the selective medias used in class - ✔✔Blood Agar

Bile Esculin

Mannitol Salt Agar (MSA)

MacConkey Agar (MaC)

Eosin Methylene Blue (EMB)

Hektoen Enteric Agar (HEA)

Xylose Lysine Desoxiolate (XLD)

Name the differential medias used in class - ✔✔O/F Glucose

MRVP

Catalase

Oxidase

Citrate

Starch

Indole

TSI

What does the acronym IMViC stand for? - ✔✔indole

Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE

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Microbiology Lab Final Exam Questions and Answers 100% Pass

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