c c c c c c c c
Edition By John W Foster, Zarrintaj Aliabadi, Joan L Slon
c c c c c c c c c
czewski
Whatciscthecconceptcofcspontaneouscgeneration?c-
cANSWERccclivingcorganismsccouldcdevelopcfromcnonlivingcorcdecomposingcmatter
Whocchallengedcthecconceptcofcspontaneouscgenerationcbycshowingcthatcmaggotsconcd
ecayingcmeatccamecfromcflies,cnotcfromcthecmeatcitself?c-cANSWERcccFrancescocRedi
Whocshowedcthatcmuttoncbrothcboiledcincflaskscandcthencsealedccouldcstillcdevelopcmicr
oorganisms,cwhichcsupportedcthectheorycofcspontaneouscgeneration?c-
cANSWERcccJohncNeedham
Whocshowedcthatcflaskscsealedcandcthencboiledchadcnocgrowthcofcmicroorganisms?c-
cANSWERcccLazzarocSpallanzani
Whocproposedcthatcairccarriedcgerms?c-cANSWERcccSpallanzani
Whocdisprovedcthectheorycofcspontaneouscgeneration?c-cANSWERcccLouiscPasteur
Whocdemonstratedcthatcdustccarriedcmicrobescandcprovedcevidencecforcthecexistenceco
fcheat-cresistantcformscofcbacteria?c-cANSWERcccJohncTyndall
Whocshowedcthatcacsilkwormcdiseasecwasccausedcbycacfungus?c-
cANSWERcccAgostinocBassi
WhocdemonstratedcthatcthecGreatcPotatocBlightcwasccausedcbycacwatercmold?c-
cANSWERcccM.cJ.cBerkely
Whocshowedcthatcthecpebrinecdiseasecofcsilkwormscwasccausedcbycacprotozoancparasit
e?c-cANSWERcccLouiscPasteur
Whocdevelopedcacsystemcofcsurgerycdesignedctocpreventcmicroorganismscfromcenterin
gcwounds?c-cANSWERcccJohncLister
Whoctransformedcthecpracticecofcsurgery?c-cANSWERcccJohncLister
WhocestablishedcthecrelationshipcbetweencBacilluscanthraciscandcanthrax?c-
cANSWERcccRobertcKoch
WhatcarecthecKochcPostulates?c-
cANSWERccc1.Thecmicroorganismscmustcbecpresentcinceveryccasecofcthecdiseasecbutcab
sentcfromchealthycindividualsc
2.Thecsuspectedcmicroorganismscmustcbecisolatedcandcgrowncincpurecculturec
,3.cThecsamecdiseasecmustcresultcwhencthecisolatedcmicroorganismciscinoculatedcintocac
healthychostc
4.cThecsamecmicroorganismcmustcbecisolatedcagaincfromcthecdiseasedchost
LimitationscofcKoch'scPostulatesc-cANSWERccc-
Somecorganismsccannotcbecgrowncincpurecculture
-Usingchumanscinccompletingcthecpostulatesciscunethical
-Molecularcandcgeneticcevidencecmaycreplace
WhatcvaccinationcdidcEdwardcJennercmake?c-cANSWERcccsmallpoxcvaccination
WhatcvaccinationscdidcPasteurcproduce?c-
cANSWERcccchickenccholera,crabiescandcanthrax
Whocinducedcformationcofcdippedctoxin-antitoxinscrabbitsc-
cANSWERcccEmilcvoncBehringcandcShibasaburocKitasato
Whocshowedcthatcthecsilkwormcdiseasecwasccausedcbycfungus?c-cANSWERcccBassi
WhocdemonstratedcthatcthecGreatcPotatocBlightcofcIrelandcwasccausedcbycacwatercmold
?c-cANSWERcccM.J.cBerkeley
Whocshowedcthatcthecperbrinecdiseasecofcsilkwormscwasccausedcbycacprotozoancparasi
te?c-cANSWERcccLouiscPasteur
Acsimplecmorphologycandclackcactruecmembrane-delimitedcnucleusc-
cANSWERcccprokaryotes
Whatciscacmorphologicallyccomplexcandchascacmembrane-enclosedcnucleus?c-
cANSWERcccEucaryotes
Whatcletctocthecdiscoverycofcvirusescascdiseaseccausingcagents?c-
cANSWERcccbacterialcfiltercthatcremovedcbacteriacandclargercmicrobesc(CharlescChamb
erland)
Thecfiveckingdomcclassificationcschemecisc-
cANSWERcccthecMoneracorcProcaryotae,cProtista,cFungi,cAnimalia,candcPlantae
Whatcarecribozymes?c-
cANSWERcccRNAcmoleculescthatcformcpeptidecbondscandcperformccellularcworkcandcrepl
ication
Thecthreecdomaincschemecisc-cANSWERcccBacteria,cArchaea,candcEucarya
Thecthreecdomaincschemeciscbasedcon?c-cANSWERcccaccomparaisoncofcribosomalcRNA
Thecearliestccellscmaychavecbeenc-cANSWERcccRNAcsurroundedcbycliposomes
Whatcarecthecthreecbacterialcmorphology?c-cANSWERccc-bacillusc(littlecrod)
-coccusc(graincorcberry)
,-spirillumc(coiledcorchelical)
Whatcarecthectypescofclightcmicroscopes?c-cANSWERccc-bright-fieldcmicroscope
-dark-fieldcmicroscope
-phase-contrastcmicroscope
-fluorescencecmicroscopes
Whatciscacmeasurecofchowcgreatlycacsubstancecslowscthecvelocitycofclight?c-
cANSWERcccRefractivecindex
Thecdirectioncandcmagnitudecofcbendingclightcincacmicroscopeciscdeterminedcbycthec___
__c-cANSWERcccrefractivecindexescofcthectwocmediacformingcthecinterface
Whichcmicroscopecproducescacdarkcimagecagainstcacbrightercbackground?c-
cANSWERcccthecbright-fieldcmicroscope
Whatciscthecproductcofcthecmagnificationscofcthecocularclenscandcthecobjectiveclens?c-
cANSWERcccthectotalcmagnification
Whatciscthectotalcmagnificationcofcthecbrightcfield,cdarkcfield,candcphase-
contrastcmicroscopes?c-cANSWERccc2000x
Whatciscthectotalcresolutioncofcthecbrightcfield,cdarkcfield,candcphaseccontrastcmicroscope
s?c-cANSWERccc0.2um
Whatciscthecpurposecofcthecbrightcfieldcmicroscope?c-
cANSWERcccforclivecandcpreservedcstainedcspecimens
Whatciscthecpurposecofcthecdarkcfieldcmicroscope?c-
cANSWERccctocobservecliving,cunstainedcpreparations
Whichcmicroscopescarecgoodcforcobservingclivingccells?c-cANSWERccc-
ThecDifferentialcInterferencecContrastcMicroscope
-Phase-contrastcmicroscope
Whichcmicroscopecenhancesctheccontrastcbetweencintracellularcstructureschavingcsligh
tcdifferencescincthecrefractivecindex?c-cANSWERcccphase-contrastcmicroscope
Whichcmicroscopecshowscacbrightcimagecofcthecobjectcresultingcfromcthecfluorescentclig
htcemittedcbycthecspecimen?c-cANSWERcccthecfluorescencecmicroscope
Thecfluorescencecmicroscopecexposescthecspecimenctocwhatctypescofclight?c-
cANSWERcccuv,cviolet,corcblue
Thecdistancecbetweenctheccentercofctheclenscandcfocalcpoint?c-cANSWERcccfocalclength
Thecshortercthecfocalcpoint,cthec______cthecmagnificationc-cANSWERcccgreater
Thecshortercthecwavelength,cthec______cthecresolutionc-cANSWERcccgreater
, Thecdistancecbetweencthecfrontcsurfacecofclenscandcsurfacecofccovercglassconcspecimen
c-cANSWERcccWorkingcdistance
Ascthecworkingcdistancecdecrease,cthecfieldcofcviewc_____c-cANSWERcccdecrease
Ascthecfieldcofcviewcdecrease,ctheclightcgetsc____c-cANSWERcccdimmer
Whatciscthecpurposecofcaddingcthecimmersioncoil?c-
cANSWERccctocobtaincmoreclightcbycprovidingcthecsamecrefractioncenergycascglass
Ascthecmagnificationcincreases,cwhatchappensctocthecfocalclength?c-
cANSWERcccitcdecrease
Whatciscthecchargecofcacbacteriaccellcsurface?c-cANSWERcccnegativeccharge
Whatciscthecchargecofcbasiccdyes?c-cANSWERcccpositiveccharge
Whatciscthecchargecofcacidcdyes?c-cANSWERcccnegativeccharge
Acthin,cuniformcfilmcofcbacterialcgrowthconcacglasscslidecincorderctocproceedcwithcfurthercs
tainingcforcmicroscopiccexaminationc-cANSWERcccsmear
Thecprocesscbycwhichcorganismcisckilledcandcfirmlycattachedctocmicroscopecslidec-
cANSWERcccfixation
Heatcfixationcvs.cChemicalcfixingc(notcthecprocedure)c-cANSWERccc-
heatcfixationcpreservescoverallcshapecbutcnotcinternalcstructures
-
chemicalcfixingcprotectscfineccellularcstructurecandcshapecofclarger,cmorecdelicatecorgani
sms
Whatcinformationcdoescdifferentialcstainingcprovide?c-
cANSWERcccdividescmicroorganismscintocgroupscbasedconctheircstainingcproperties
Whatciscthecmostcwidelycusedcdifferentialcstainingcprocedure?c-
cANSWERcccgramcstaining
Negativecstainingc-cANSWERcccthecbacteriaciscnotcstained;cthecbackgroundciscstained
Whatciscshadowingcincelectroncmicroscopy?c-
cANSWERccccoatingcspecimencwithcacthincfilmcof cheavycmetal
WhatciscthectotalcmagnificationcofcthecTransmissioncElectroncMicroscopecandcthecScann
ingcElectroncMicroscope?c-cANSWERccc1,000,000x
WhatciscthectotalcmagnificationcofcthecScanningcElectroncMicroscope?c-
cANSWERccc1,000,000x
Whichcmicroscopecusescelectronscreflectedcfromcthecsurfacecofcacspecimenctoccreateca
ncimage?c-cANSWERcccscanningcelectroncmicroscope