BIOCHEM 4300 EXAM 2 QUESTIONS WITH CORRECT
DETAILED ANSWERS (A+) 100% VERIFIED
What describes the ligand binding states of the entire macromolecule?
Partition Function (Q)
What describes the ligand binding states of specific binding sites?
Binding Polynomial (P)
What is the role of Myoglobin?
Carries O2
What is the role of Hemeglobin?
Loads O2 in the lungs and unloads it in the body
When does K increase in cooperative binding?
When the number of ligands bound increases.
ΔGc is positive when...
There is Positive Cooperativity
ΔGc is negative when...
There is Negative Cooperativity
Why can you not determine K from negative coopertivity?
Negative cooperativity can not be distinguished from non identical binding sites.
,What do you do to determine the coupling energy?
Find the gibbs free energy of the reaction and the average of the gibbs in both reactions.
What are the possible ways one could determine the potential K in a non identical
binding site?
Inactivate one of the binding sites through Mutagenesis.
Study single M fragments of the Ligand.
What is the difference between homotropic and heterotropic cooperative binding
Homotropic - A molecule of X binds to the ligand and aids in a secondary binding of the
molecule X. (XX)
Heterotrophic - A molecule of X binds to the ligand and aids in a secondary binding of
molecule Y. (XY)
What are the two different ways that ligand binding data can be obtained?
Direct Methods: measure the direct conc. of each component in an equilibrated mixture.
Indirect Methods: Inferred from a change of a property of the ligand or macromolecule.
, What is a Bronstead Acid?
HA, Proton Donor
What is a Bronstead Base?
B, Proton Acceptor
What is the Autoprotolysis reaction for water?
2H20 (l) = H3O+ (aq) + OH- (aq)
Weak acids are equal to (Ka value and pKa value)
Ka < 1 , pKa > 0
How do you turn ka into pKa?
pKa = -log(Ka)
How do you solve for Kb given a solution (Bascisity Constant)?
B(aq) + H2O(l) <-> HB+ (aq) + OH- (aq)
Kb = [HB][OH-]/[B]
DETAILED ANSWERS (A+) 100% VERIFIED
What describes the ligand binding states of the entire macromolecule?
Partition Function (Q)
What describes the ligand binding states of specific binding sites?
Binding Polynomial (P)
What is the role of Myoglobin?
Carries O2
What is the role of Hemeglobin?
Loads O2 in the lungs and unloads it in the body
When does K increase in cooperative binding?
When the number of ligands bound increases.
ΔGc is positive when...
There is Positive Cooperativity
ΔGc is negative when...
There is Negative Cooperativity
Why can you not determine K from negative coopertivity?
Negative cooperativity can not be distinguished from non identical binding sites.
,What do you do to determine the coupling energy?
Find the gibbs free energy of the reaction and the average of the gibbs in both reactions.
What are the possible ways one could determine the potential K in a non identical
binding site?
Inactivate one of the binding sites through Mutagenesis.
Study single M fragments of the Ligand.
What is the difference between homotropic and heterotropic cooperative binding
Homotropic - A molecule of X binds to the ligand and aids in a secondary binding of the
molecule X. (XX)
Heterotrophic - A molecule of X binds to the ligand and aids in a secondary binding of
molecule Y. (XY)
What are the two different ways that ligand binding data can be obtained?
Direct Methods: measure the direct conc. of each component in an equilibrated mixture.
Indirect Methods: Inferred from a change of a property of the ligand or macromolecule.
, What is a Bronstead Acid?
HA, Proton Donor
What is a Bronstead Base?
B, Proton Acceptor
What is the Autoprotolysis reaction for water?
2H20 (l) = H3O+ (aq) + OH- (aq)
Weak acids are equal to (Ka value and pKa value)
Ka < 1 , pKa > 0
How do you turn ka into pKa?
pKa = -log(Ka)
How do you solve for Kb given a solution (Bascisity Constant)?
B(aq) + H2O(l) <-> HB+ (aq) + OH- (aq)
Kb = [HB][OH-]/[B]
