DAT LAB TEST 7 QUESTIONS AND
ANSWERS
what is the purpose of performing the DAT procedure? - Answer - used to determine if red cells have
been coated in vivo with immunoglobulin, complement or both.
what does a positive DATE indicate? - Answer - indicates that red cells are coated with protein in-vivo
what 3 reagents may be used in the DAT procedure? - Answer - polyspecific IgG, C3d
monospecific IgG or C3d
what is the sample of choice for the DAT? Why? - Answer - whole blood collected in an EDTA tube
because it prevents in-vitro fixation of complement.
the DAT is used to assist in hemolysis associated with what 4 clinical conditions: - Answer - HDFN
autoimmune hemolytic anemia
red cell sensitization
transfusion rxns
what 3 items in the pt history are crucial in determining the clinical significance of a positive DAT? -
Answer - pts diagnosis
transfusion and pregnancy hx
recent/current medications
state the principle of the DAT procedure - Answer - the detection of abs that attached to red cells in-
vivo
what ab class is involved in HDFN? - Answer - IgG
ANSWERS
what is the purpose of performing the DAT procedure? - Answer - used to determine if red cells have
been coated in vivo with immunoglobulin, complement or both.
what does a positive DATE indicate? - Answer - indicates that red cells are coated with protein in-vivo
what 3 reagents may be used in the DAT procedure? - Answer - polyspecific IgG, C3d
monospecific IgG or C3d
what is the sample of choice for the DAT? Why? - Answer - whole blood collected in an EDTA tube
because it prevents in-vitro fixation of complement.
the DAT is used to assist in hemolysis associated with what 4 clinical conditions: - Answer - HDFN
autoimmune hemolytic anemia
red cell sensitization
transfusion rxns
what 3 items in the pt history are crucial in determining the clinical significance of a positive DAT? -
Answer - pts diagnosis
transfusion and pregnancy hx
recent/current medications
state the principle of the DAT procedure - Answer - the detection of abs that attached to red cells in-
vivo
what ab class is involved in HDFN? - Answer - IgG
