Biomed EOC Review Exam Questions And
Correct Answers (A+)
Which of the following are roles of the p53 gene? - ANSWER stop cell cycle
repairs DNA
starts apoptosis
DNA microarrays measure - ANSWER the amount of mRNA for every gene that is
expressed
_____ have surface ______ where _____ attach - ANSWER pathogens, antigens,
antibodies
The following DNA sequence is from a person who has limited side effects from an
experimental cancer drug.
AATTCTAGGACCTGC - ANSWER AATTCTCGGAGCTGC
Why type of nanotechnology detects the presence of proteins that are released by
cancer cells - ANSWER nanoscale cantilevers
Which of the following diagnostic imaging technique uses non-ionizing radiation -
ANSWER MRI
A couple comes to the genetic counselling Center. As a counselor here, you will advise
them of their options. Their first son has x-linked recessive diease. This couple is
concerned that they might have a second child affected with the same x-linked
recessive disease. Neither of the parents displays the trait. What would you tell them? -
ANSWER they have a 25% chance of having another boy with the disorder
,What are key successful gene delivery - ANSWER target the right cells
integrate the gene in the cells
avoiding harmful side effects
_____ value is the measure of a patients level of sensitization to donor antigens -
ANSWER PRA
Hydrophobic side-chains are typically folded on the - ANSWER inferior portions of a
protein
A bacteria contains a DNA plasmid. It comes into direct contact with another bacteria.
The first bacteria give give its DNA plasmid to the second bacteria. This is an example of
- ANSWER conjugation
In HLA testing, a six-antigen match is the best compatibility between a donor and
recipient. How often does this occur within siblings who have the same mother and
father - ANSWER 25%
During the ELISA test the secondary antibody was conjugated with an enzyme. What
happens when the enzyme attaches to a substrate - ANSWER the substrate causes the
enzyme to oxidize resulting in a color change
Which bacterial transformation step increases cell membrane permeability - ANSWER
heat shock
Two patients have the same type of cancer have agreed to participate inn a clinical trial.
Microarray technology is used to identify any genes that are involved in this cancer. Of 6
genes that are analyzed, two showed a correlation of 2.3. What is the significance of
these two genes? - ANSWER the genes are both suppressed in tumor formation
Cancer is caused by an interruption of the cell cycle. Which of the following situations
would most likely produce cancer - ANSWER increased expression by oncogenes and
decreasedA expression by tumor suppressor genes
,A patient enters a hospital after hitting her head in a car accident. She is diagnosed with
a fractured skull after an x-ray. She has other symptoms which lead the doctors to
believe she may have brain damage. Which diagnostic imaging technique would be best
to diagnose the possible brain damage - ANSWER CT scan
In order to test positive for PKU (phenylketonuria); a baby must inherit two copies of the
defective gene. This genetic disorder is classified as - ANSWER recessive because two
copies of the PKU must be inherited
Why would a doctor not prescribe an antibiotic for a viral infection - ANSWER the
bacteria have more opportunity to become resistant
Which of the following antibiotics inhibits enzymes involved with the final steps of cell
wall synthesis - ANSWER b-lactam
During the final stages of hydrophobic interaction chromatography different buffer
solutions were used with different salt concentration. Why are the different buffers used
to purify the GFP? - ANSWER the buffer solution causes the hydrophobic side chains to
move and become attracted to the hydrophobic matrix in the HI column
Otosclerosis causes partial hearing loss. What part of the ear does this affect - ANSWER
middle ear
How does the adeno-associated virus carry its genetic information - ANSWER single
stranded DNA
During the final stage of PCR the DNA samples are heated to 95 degrees celsius. Why is
that the temperature important for this stage - ANSWER the temperature allows the DNA
strand to denature exposing nucleotides
A student runs an experiment using a petri dish with agar containing the antibiotic
streotomycini. The student streaks the dish with two different strains of staphylococcus
, aureus. Both strains do not grow on the dish. What is the best explanation for these
results? - ANSWER both are susceptible
A patient has undergone some hearing tests. They have normal audiograms but had
difficulty hearing with a lot of background noises. What interventions would most likely
be appropriate for this patient? - ANSWER Hearing aid to assist with filtering
background noise
Which bacterial transformation procedure step neutralizes the negative charge on both
the DNA and phospholipids - ANSWER calcium chloride treatments
Which of the following would be an example of gene therapy technology - ANSWER the
development of a nasal spray that contains copies of the normal gene that is defective in
persons with cystic fibrosis
What are characteristics of cancer cells when grown in vitro - ANSWER cancers cells
can proliferate indefinitely in vitro
cancer cells have various shapes
cancer cells have abnormal numbers of chromosomes
A bacterial culture is several days old. Many of the bacterial cells have ruptured and
released DNA into the nutrient agar. A few living bacteria cells in the culture have
become genetically enhanced by taking this DNA into the cytoplasm and are now
resistant to ampicillin. The above explains which process - ANSWER transduction
A technique where the gene mutation is analyzed using a genetic marker instead of
analyzing the gene itself - ANSWER marker analysis
50 uL of a sample is pipetted into 100 uL dilution tube. The concentration of the tube is
now 500 ng/mL. If you do a serial dilution adding 50 uL sample into 100 uL dilution tube,
into three more tubes, what is the final concentration of the last tube? - ANSWER 18.52
ng/ml
Correct Answers (A+)
Which of the following are roles of the p53 gene? - ANSWER stop cell cycle
repairs DNA
starts apoptosis
DNA microarrays measure - ANSWER the amount of mRNA for every gene that is
expressed
_____ have surface ______ where _____ attach - ANSWER pathogens, antigens,
antibodies
The following DNA sequence is from a person who has limited side effects from an
experimental cancer drug.
AATTCTAGGACCTGC - ANSWER AATTCTCGGAGCTGC
Why type of nanotechnology detects the presence of proteins that are released by
cancer cells - ANSWER nanoscale cantilevers
Which of the following diagnostic imaging technique uses non-ionizing radiation -
ANSWER MRI
A couple comes to the genetic counselling Center. As a counselor here, you will advise
them of their options. Their first son has x-linked recessive diease. This couple is
concerned that they might have a second child affected with the same x-linked
recessive disease. Neither of the parents displays the trait. What would you tell them? -
ANSWER they have a 25% chance of having another boy with the disorder
,What are key successful gene delivery - ANSWER target the right cells
integrate the gene in the cells
avoiding harmful side effects
_____ value is the measure of a patients level of sensitization to donor antigens -
ANSWER PRA
Hydrophobic side-chains are typically folded on the - ANSWER inferior portions of a
protein
A bacteria contains a DNA plasmid. It comes into direct contact with another bacteria.
The first bacteria give give its DNA plasmid to the second bacteria. This is an example of
- ANSWER conjugation
In HLA testing, a six-antigen match is the best compatibility between a donor and
recipient. How often does this occur within siblings who have the same mother and
father - ANSWER 25%
During the ELISA test the secondary antibody was conjugated with an enzyme. What
happens when the enzyme attaches to a substrate - ANSWER the substrate causes the
enzyme to oxidize resulting in a color change
Which bacterial transformation step increases cell membrane permeability - ANSWER
heat shock
Two patients have the same type of cancer have agreed to participate inn a clinical trial.
Microarray technology is used to identify any genes that are involved in this cancer. Of 6
genes that are analyzed, two showed a correlation of 2.3. What is the significance of
these two genes? - ANSWER the genes are both suppressed in tumor formation
Cancer is caused by an interruption of the cell cycle. Which of the following situations
would most likely produce cancer - ANSWER increased expression by oncogenes and
decreasedA expression by tumor suppressor genes
,A patient enters a hospital after hitting her head in a car accident. She is diagnosed with
a fractured skull after an x-ray. She has other symptoms which lead the doctors to
believe she may have brain damage. Which diagnostic imaging technique would be best
to diagnose the possible brain damage - ANSWER CT scan
In order to test positive for PKU (phenylketonuria); a baby must inherit two copies of the
defective gene. This genetic disorder is classified as - ANSWER recessive because two
copies of the PKU must be inherited
Why would a doctor not prescribe an antibiotic for a viral infection - ANSWER the
bacteria have more opportunity to become resistant
Which of the following antibiotics inhibits enzymes involved with the final steps of cell
wall synthesis - ANSWER b-lactam
During the final stages of hydrophobic interaction chromatography different buffer
solutions were used with different salt concentration. Why are the different buffers used
to purify the GFP? - ANSWER the buffer solution causes the hydrophobic side chains to
move and become attracted to the hydrophobic matrix in the HI column
Otosclerosis causes partial hearing loss. What part of the ear does this affect - ANSWER
middle ear
How does the adeno-associated virus carry its genetic information - ANSWER single
stranded DNA
During the final stage of PCR the DNA samples are heated to 95 degrees celsius. Why is
that the temperature important for this stage - ANSWER the temperature allows the DNA
strand to denature exposing nucleotides
A student runs an experiment using a petri dish with agar containing the antibiotic
streotomycini. The student streaks the dish with two different strains of staphylococcus
, aureus. Both strains do not grow on the dish. What is the best explanation for these
results? - ANSWER both are susceptible
A patient has undergone some hearing tests. They have normal audiograms but had
difficulty hearing with a lot of background noises. What interventions would most likely
be appropriate for this patient? - ANSWER Hearing aid to assist with filtering
background noise
Which bacterial transformation procedure step neutralizes the negative charge on both
the DNA and phospholipids - ANSWER calcium chloride treatments
Which of the following would be an example of gene therapy technology - ANSWER the
development of a nasal spray that contains copies of the normal gene that is defective in
persons with cystic fibrosis
What are characteristics of cancer cells when grown in vitro - ANSWER cancers cells
can proliferate indefinitely in vitro
cancer cells have various shapes
cancer cells have abnormal numbers of chromosomes
A bacterial culture is several days old. Many of the bacterial cells have ruptured and
released DNA into the nutrient agar. A few living bacteria cells in the culture have
become genetically enhanced by taking this DNA into the cytoplasm and are now
resistant to ampicillin. The above explains which process - ANSWER transduction
A technique where the gene mutation is analyzed using a genetic marker instead of
analyzing the gene itself - ANSWER marker analysis
50 uL of a sample is pipetted into 100 uL dilution tube. The concentration of the tube is
now 500 ng/mL. If you do a serial dilution adding 50 uL sample into 100 uL dilution tube,
into three more tubes, what is the final concentration of the last tube? - ANSWER 18.52
ng/ml
