biosafety levels Test | Questions with
Answers Verified 100% Correct
BSL 1 - ✔✔microorganisms not known to cause disease in healthy adults; standard
microbiological practices are followed, PPE is not required, a sink must be available for hand
washing; examples are bacillus subtilis, serratia marcescens, staphylococcus saprophyticus
BSL 2 - ✔✔indigenous microorganisms that can lead to diseases of varying severity in
healthy adults; BSL 1 procedures plus lab access is restricted, PPE must be worn including lab
coats, gloves, and eye protection, work that may generate aerosols or splashes are performed
in a biological safety cabinet, contaminated waste is autoclaved, the lab has self closing doors, a
sink and eye wash are available; examples: s. aureus, streptococcus pyogenes, and salmonella
BSL 3 - ✔✔indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission; BSL 2 procedures plus medical surveillance of lab
employees, immunizations may be required; locking, double door access separates the labs
work area from the other part of the facility, PPL must be worn including lab coats, gloves, eye
protection, and respirators; all work is performed in biosafety cabinet, decontamination of all
waste and lab clothing before laundering; lab must have negative air flow, exhaust air cannot
be recirculated; hands free sink and eye wash must be available; examples: mycobacterium
tuberculosis, bacillus anthracis, francisella tularensis
Answers Verified 100% Correct
BSL 1 - ✔✔microorganisms not known to cause disease in healthy adults; standard
microbiological practices are followed, PPE is not required, a sink must be available for hand
washing; examples are bacillus subtilis, serratia marcescens, staphylococcus saprophyticus
BSL 2 - ✔✔indigenous microorganisms that can lead to diseases of varying severity in
healthy adults; BSL 1 procedures plus lab access is restricted, PPE must be worn including lab
coats, gloves, and eye protection, work that may generate aerosols or splashes are performed
in a biological safety cabinet, contaminated waste is autoclaved, the lab has self closing doors, a
sink and eye wash are available; examples: s. aureus, streptococcus pyogenes, and salmonella
BSL 3 - ✔✔indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission; BSL 2 procedures plus medical surveillance of lab
employees, immunizations may be required; locking, double door access separates the labs
work area from the other part of the facility, PPL must be worn including lab coats, gloves, eye
protection, and respirators; all work is performed in biosafety cabinet, decontamination of all
waste and lab clothing before laundering; lab must have negative air flow, exhaust air cannot
be recirculated; hands free sink and eye wash must be available; examples: mycobacterium
tuberculosis, bacillus anthracis, francisella tularensis
