BIO 219 QUIZZES Questions With Complete
Solutions
Which gene in the lux operon encodes the auto-inducer, AHL
Lux R
b.
Lux I
c.
Lux A
d.
Lux D b
What is the overall purpose of the quarter-long project?
I. To map the lux operon
II. To isolate the lux operon from A. fischeri and transform E. coli with it the operon to induce
bioluminescence
III. To induce the upregulation of the gadA gene in E. coli through quorum sensing 2 and
3
,BIO 219 QUIZZES Questions With Complete
Solutions
Regulation of gene expression in response to changes in cell population density is known as
_______________________. quorum sensing
If you wanted to get a brighter bioluminescence from your culture of Allivibrio which of the
following "treatments" would increase the amount of blue-green light emitted?
treatment with CO2
b.
Treatment with chaotropic salts
c.
treatment with O2
d.
treatment with acid
e.
Treatment with a alkaline buffer c
What is used to elute the DNA from the silica membrane in the spin column?
a.
,BIO 219 QUIZZES Questions With Complete
Solutions
Chaotropic salts
b.
SDS (sodium dodecy sulfate)
c.
Water
d.
Ethanol
e.
A low pH buffer. c
Good minions always wear their [x] and [y] for lab safety! gloves
goggles
What is the range for the 260:280 ratio that indicates a DNA sample of "good purity"?
2.2 - 3.5
b.
0.5 - 1.5
c.
, BIO 219 QUIZZES Questions With Complete
Solutions
2.0 - 2.2
d.
1.5 - 1.8
e.
1.8 - 2.0 e
Of the assignments listed in the answers, which one is NOT done as group work?
Final Exam
b.
Lab Report 1
c.
Lab Notebooks
d.
Lab Report 2 final exam
A 260:280 ratio = 2.36 would indicate protein contamination. false
Solutions
Which gene in the lux operon encodes the auto-inducer, AHL
Lux R
b.
Lux I
c.
Lux A
d.
Lux D b
What is the overall purpose of the quarter-long project?
I. To map the lux operon
II. To isolate the lux operon from A. fischeri and transform E. coli with it the operon to induce
bioluminescence
III. To induce the upregulation of the gadA gene in E. coli through quorum sensing 2 and
3
,BIO 219 QUIZZES Questions With Complete
Solutions
Regulation of gene expression in response to changes in cell population density is known as
_______________________. quorum sensing
If you wanted to get a brighter bioluminescence from your culture of Allivibrio which of the
following "treatments" would increase the amount of blue-green light emitted?
treatment with CO2
b.
Treatment with chaotropic salts
c.
treatment with O2
d.
treatment with acid
e.
Treatment with a alkaline buffer c
What is used to elute the DNA from the silica membrane in the spin column?
a.
,BIO 219 QUIZZES Questions With Complete
Solutions
Chaotropic salts
b.
SDS (sodium dodecy sulfate)
c.
Water
d.
Ethanol
e.
A low pH buffer. c
Good minions always wear their [x] and [y] for lab safety! gloves
goggles
What is the range for the 260:280 ratio that indicates a DNA sample of "good purity"?
2.2 - 3.5
b.
0.5 - 1.5
c.
, BIO 219 QUIZZES Questions With Complete
Solutions
2.0 - 2.2
d.
1.5 - 1.8
e.
1.8 - 2.0 e
Of the assignments listed in the answers, which one is NOT done as group work?
Final Exam
b.
Lab Report 1
c.
Lab Notebooks
d.
Lab Report 2 final exam
A 260:280 ratio = 2.36 would indicate protein contamination. false
