Bio 226 Enzymes Lab Report

Laboratory Report



LABORATORY REPORT
Activity: Enzyme Activity
Name: Theresa Nichole McDaris
Instructor: Adrea Kelley
Date: 07.23.2021

Predictions
1. Sucrase will have the greatest activity at
pH 3


2. Sucrase will have the greatest activity at
40 °C (104 °F)


3. Sucrase activity
increases with increasing sucrose concentration until a plateau is reached



Materials and Methods
Effect of pH on Enzyme Activity
1. Dependent Variable
amount of product (glucose and fructose) produced


2. Independent Variable
pH


3. Controlled Variables
temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time



Effect of Temperature on Enzyme Activity
1. Dependent Variable
amount of product (glucose and fructose) produced


2. Independent Variable
temperature


3. Controlled Variables
pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time



Effect of Substrate Concentration on Enzyme Activity
1. Dependent Variable
amount of product (glucose and fructose) produced


2. Independent Variable
amount of substrate (sucrose) present


3. Controlled Variables
temperature, pH, sucrase + sucrose incubation time


4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity.
Glucose and fructose will be produced only in presence of sucrase activity and more the sucrase activity, more the glucose and fructose
produced.




Laboratory Report/ Theresa Nichole McDaris/ Enzyme Activity/ Adrea Kelley/ 07.23.2021/ Page [1] of [4]